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Assigning biological functions to rice genes by genome annotation, expression analysis and mutagenesis

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Abstract

Rice is the first cereal genome to be completely sequenced. Since the completion of its genome sequencing, considerable progress has been made in multiple areas including the whole genome annotation, gene expression profiling, mutant collection, etc. Here, we summarize the current status of rice genome annotation and review the methodology of assigning biological functions to hundreds of thousands of rice genes as well as discuss the major limitations and the future perspective in rice functional genomics. Available data analysis shows that the rice genome encodes around 32,000 protein-coding genes. Expression analysis revealed at least 31,000 genes with expression evidence from full-length cDNA/EST collection or other transcript profiling. In addition, we have summarized various strategies to generate mutant population including natural, physical, chemical, T-DNA, transposon/retrotransposon or gene silencing based mutagenesis. Currently, more than 1 million of mutants have been generated and 27,551 of them have their flanking sequence tags. To assign biological functions to hundreds of thousands of rice genes, global co-operations are required, various genetic resources should be more easily accessible and diverse data from transcriptomics, proteomics, epigenetics, comparative genomics and bioinformatics should be integrated to better understand the functions of these genes and their regulatory mechanisms.

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Acknowledgements

We thank Drs. Ildiko Szeverenyi, Tatiana Kolesnik and Doris Bachmann as well as Zhigang Ma and Hongfen Luan for their help in the generation and investigation of Ds insertion lines.

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  1. Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore, 117604, Singapore

    Shu-Ye Jiang & Srinivasan Ramachandran

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  2. Srinivasan Ramachandran
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Correspondence to Srinivasan Ramachandran.

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Jiang, SY., Ramachandran, S. Assigning biological functions to rice genes by genome annotation, expression analysis and mutagenesis. Biotechnol Lett 32, 1753–1763 (2010). https://doi.org/10.1007/s10529-010-0377-7

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