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Acetate accumulation through alternative metabolic pathways in ackA pta poxB triple mutant in E. coli B (BL21)

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Abstract

Individual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3.1 and 1.7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA-pta mutants did not change the acetate accumulation pattern. When the acetate production genes (ackA-pta-poxB) were deleted in E. coli B (BL21) acetate still accumulated. This may be an indication that perhaps acetate is not only a by-product of carbon metabolism; it is possible that acetate plays also a role in other cellular metabolite pathways. It is likely that there are alternative acetate production pathways.

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Acknowledgments

Funding was provided by the Intramural program at the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health. The authors would like to thank D. Livnat for proof reading of the manuscript.

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Correspondence to Joseph Shiloach.

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Purpose of work

Compared with E. coli K-12, E. coli B (BL21) is low acetate producer. To identify new ways to decrease acetate in E. coli K-12, the effect of deleting genes involved in the production and consumption of acetate in E. coli B (BL21) was evaluated.

Je-Nie Phue, Sang Jun Lee, Equal contribution with 1st author.

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Phue, JN., Lee, S.J., Kaufman, J.B. et al. Acetate accumulation through alternative metabolic pathways in ackA pta poxB triple mutant in E. coli B (BL21). Biotechnol Lett 32, 1897–1903 (2010). https://doi.org/10.1007/s10529-010-0369-7

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  • DOI: https://doi.org/10.1007/s10529-010-0369-7

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