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Biotechnology Letters

, Volume 32, Issue 6, pp 781–786 | Cite as

Novel method to obtain highly enriched cultures of adult rat Schwann cells

  • Ali Niapour
  • Fereshteh Karamali
  • Khadijeh Karbalaie
  • Abbas Kiani
  • Mohammad Mardani
  • Mohammad Hossein Nasr-EsfahaniEmail author
  • Hossein BaharvandEmail author
Original Research Paper

Abstract

Schwann cells (SCs) can be used to repair both the peripheral and central nervous systems. Therefore, establishment of a procedure to obtain activated, highly proliferative SCs, in an appropriate time for clinical applications, is a prerequisite. Purification is complicated by contamination with fibroblasts which often become the predominant cell type in an in vitro SC culture. This study describes a novel and efficient method to enrich SCs by utilizing the differential detachment properties of the two cell types. In culture, cells were treated with two different media and the chelator, EGTA, which detached SCs faster than fibroblasts and allowed for easy isolation of SCs. Within seven days, high yields of SCs with a purity of greater than 99% were achieved. This was confirmed by immunostaining characterization and flow-cytometric analyses using an antibody against the p75 low affinity nerve growth factor receptor (p75LNGFR). The entire procedure was completed in approximately 21 days. This method has the advantage of being technically easier, faster, and more efficient than other previously described methods. An SC culture that was about 99% homogenous was achieved.

Keywords

Sciatic nerve Predegeneration Schwann cell Detachment property Purification 

Notes

Acknowledgment

This study was supported by a grant from Royan Institute, Iran.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ali Niapour
    • 1
    • 2
  • Fereshteh Karamali
    • 1
  • Khadijeh Karbalaie
    • 1
  • Abbas Kiani
    • 1
  • Mohammad Mardani
    • 2
  • Mohammad Hossein Nasr-Esfahani
    • 1
    Email author
  • Hossein Baharvand
    • 3
    • 4
    Email author
  1. 1.Department of Cell and Molecular BiologyRoyan Institute for Animal Biotechnology, ACECRIsfahanIran
  2. 2.Department of AnatomyIsfahan University of Medical ScienceIsfahanIran
  3. 3.Department of Stem Cells and Developmental BiologyRoyan Institute for Stem Cell Biology and Technology, ACECRTehranIran
  4. 4.Department of Developmental BiologyUniversity of Science and Culture, ACECRTehranIran

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