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Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum

Biotechnology Letters volume 32, pages 695–699 (2010)Cite this article

Abstract

Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM≈1 g l−1) where the bacterium was grown for 24 h, at 30°C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg−1). The growing cells produced 0.076 g ADD from 1 g cholesterol l−1.

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Affiliations

  1. School of Life Sciences, North Maharashtra University, P B 80, Jalgaon, 425 001, India

    Priti N. Chaudhari, Bhushan L. Chaudhari & Sudhir B. Chincholkar

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  1. Priti N. Chaudhari
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  2. Bhushan L. Chaudhari
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  3. Sudhir B. Chincholkar
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Correspondence to Bhushan L. Chaudhari.

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Chaudhari, P.N., Chaudhari, B.L. & Chincholkar, S.B. Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum . Biotechnol Lett 32, 695–699 (2010). https://doi.org/10.1007/s10529-010-0206-z

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  • DOI: https://doi.org/10.1007/s10529-010-0206-z

Keywords

  • Androsta-1,4-diene-3,17-dione
  • Biotransformation
  • Cholesterol
  • Chryseobacterium gleum