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Biotechnology Letters

, Volume 31, Issue 7, pp 953–957 | Cite as

HPLC analysis of lipoproteins in culture medium of hepatoma cells: an in vitro system for screening antihyperlipidemic drugs

  • Mizuho Itoh
  • Yukie Abe
  • Yuka Iwama
  • Fumiko Kimura
  • Mayumi Satoh
  • Mayumi Shoji
  • Junichiro Takahashi
  • Gen Toshima
  • Hiroki Sasaki
  • Kazuyuki Hiwatashi
  • Keishi Hata
Original Research Paper

Abstract

We isolated a HepG2-derived sub-clone (HepG2-Lipo), which possessed an increased lipoprotein synthesizing ability. HepG2-Lipo cells could secrete triglycerides (TG) and cholesterol at rates 9.4- and 6-fold higher, respectively, when compared to HepG2 cells. Real-time RT-PCR analysis revealed that the expression levels of sterol regulatory element-binding protein-1c and -2 were 2.9- and 1.5-fold higher than in HepG2 cells. Furthermore, two apolipoprotein (apo) genes (apoA-1 and apoB-100) in HepG2-Lipo cells were expressed at 2.8- and 1.9-fold higher levels when compared to those in parental cells. We examined the effects of three antihyperlipidemic agents on the lipoprotein profiles of HepG2-Lipo cells. Simvastatin at 5 μM selectively suppressed cholesterol secretion from HepG2-Lipo cells, and 500 μM fenofibrate inhibited both TG and cholesterol secretion from the cells.

Keywords

Antihyperlipidemic agents Apolipoprotein Cholesterol HepG2 cells Lipoprotein profile Triglyceride 

Notes

Acknowledgement

This research was supported in part by the grant program “Research for Promoting Technological Seeds” from the Japan Science and Technology Agency.

References

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Mizuho Itoh
    • 1
  • Yukie Abe
    • 1
  • Yuka Iwama
    • 1
  • Fumiko Kimura
    • 1
  • Mayumi Satoh
    • 1
  • Mayumi Shoji
    • 1
  • Junichiro Takahashi
    • 1
  • Gen Toshima
    • 1
  • Hiroki Sasaki
    • 1
  • Kazuyuki Hiwatashi
    • 2
  • Keishi Hata
    • 2
  1. 1.Skylight Biotech Inc.AkitaJapan
  2. 2.Institute for Food and BrewingAkita Prefectural Agricultural, Forestry, and Fisheries Research CenterAkitaJapan

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