Abstract
A protease was purified from Bacillus sp. DJ isolated from Doenjang, a traditional Korean fermented food. Its molecular weight (MW) and isoelectric point (pI) were 18-19 kDa and 6.0–6.5 using 1- or 2-D fibrin zymography, respectively. The protease was optimally active at pH 9 and 55°C. Activity was inhibited by 1 mM PMSF, but not by EDTA, EGTA, aprotinin, or leupeptin, indicating that the protease is a serine protease. By using a new electrophoretic technique, multiple loading of O’Farrell-type isoelectric focusing (IEF) slab gel, the first amino acid residues of the N-terminal sequence of the protease were determined as HPLVLVDPIL, which is 80% identical with serine proteases of the subtilase family.
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Acknowledgments
This work was supported by a high throughput screening (HTS)-based Integrated Technology Development Grant (No. 2008-04171) from the Ministry of Education, Science and Technology through the Korea Science and Engineering Foundation, and a basic research grant from KRIBB.
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Choi, NS., Choi, J.H., Yoon, JH. et al. Identification of a serine protease from a Bacillus sp. using multiple loading of O’Farrell-type isoelectric focusing slab two-dimensional gel. Biotechnol Lett 31, 975–978 (2009). https://doi.org/10.1007/s10529-009-9962-z
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DOI: https://doi.org/10.1007/s10529-009-9962-z