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Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition

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Abstract

The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34+ phenotypic analysis with flow cytometry. At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34+ cells had achieved 18.7 ± 1.6, 11.6 ± 0.9 and 23.4 ± 2-fold expansions, respectively, in the test groups. These were significantly different from those in control groups. Microencapsulated osteoblasts under hypoxia conditions had therefore a significant effect on the expansion potential of HSPCs in vitro.

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Acknowledgments

This work was supported by grants from the National Natural Sciences Foundation of China (30700181) and the New teacher foundation of Ministry of Education (20070141055).

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Authors and Affiliations

  1. Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China

    Kedong Song, Guofeng Zhao, Tianqing Liu, Lei Zhang & Xuehu Ma

  2. Department of Neuromedicine, First Affiliated Hospital, Dalian Medical University, Dalian, 116021, China

    Jing Liu

  3. Department of Engineering Science, Oxford University, Parks Road, Oxford, OX1 3PJ, UK

    Zhanfeng Cui

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  1. Kedong Song
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  2. Guofeng Zhao
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  3. Tianqing Liu
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  4. Lei Zhang
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  5. Xuehu Ma
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  6. Jing Liu
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  7. Zhanfeng Cui
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Correspondence to Tianqing Liu.

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Song, K., Zhao, G., Liu, T. et al. Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition. Biotechnol Lett 31, 923–928 (2009). https://doi.org/10.1007/s10529-009-9961-0

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  • DOI: https://doi.org/10.1007/s10529-009-9961-0

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