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Anti-inflammatory effects of talosin A via inhibition of NF-kappaB activation in lipopolysaccharide-stimulated RAW264.7 cells

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Abstract

Aim of work To determine whether talosin A inhibits production of pro-inflammatory cytokines and nitric oxide (NO) in lipopolysaccharide (1 μg/ml)-stimulated RAW 264.7 macrophages. Talosin A (10 and 50 μg/ml) significantly reduced LPS-induced overproduction of tumor necrosis factor-α, interleukin IL-1β, -6 and NO in a dose-dependent manner (P < 0.01). Talosin A had a direct NO-scavenging activity in the cell-free system. In RT-PCR analysis, gene expressions were inhibited at a transcriptional level. Moreover, the activation of nuclear factor-kappa B (NF-κB) was significantly suppressed by talosin A in LPS-stimulated macrophage cells (P < 0.05). Therefore, we confirmed anti-inflammatory activity of talosin A was via suppression of pro-inflammatory cytokines, NO production and NF-κB activation, suggesting a therapeutic candidate for inflammatory disorders.

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Acknowledgement

This work was partially supported by a grant code #200702186 from the BioGreen 21 Program, Rural Development Administration, the Republic of Korea.

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Authors and Affiliations

  1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon, 305-764, Republic of Korea

    Youn-Hwan Hwang, Myoung-Seok Kim, In-Bae Song & Hyo-In Yun

  2. B&C Biopharm, 906-5 Iui-dong, Yeongtong-gu, Suwon, Republic of Korea

    Jong-Hwan Lim

  3. JUNJINBIO, 711 Hosan-dong, Dalseo-gu, Daegu, Republic of Korea

    Byung-Kwon Park

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  1. Youn-Hwan Hwang
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  2. Myoung-Seok Kim
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  3. In-Bae Song
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  4. Jong-Hwan Lim
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  5. Byung-Kwon Park
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  6. Hyo-In Yun
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Correspondence to Hyo-In Yun.

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Hwang, YH., Kim, MS., Song, IB. et al. Anti-inflammatory effects of talosin A via inhibition of NF-kappaB activation in lipopolysaccharide-stimulated RAW264.7 cells. Biotechnol Lett 31, 789–795 (2009). https://doi.org/10.1007/s10529-009-9943-2

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  • DOI: https://doi.org/10.1007/s10529-009-9943-2

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