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Enzymatic enrichment of egg-yolk phosphatidylcholine with α-linolenic acid

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Abstract

α-Linolenic acid (ALA) was incorporated at 28% into the sn-1 position of egg-yolk phospatidylcholine using Novozyme 435 in one-step transesterification process. Using phospholipase A2 in a two-step process gave 25% incorporation of ALA into the sn-2 position.

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References

  • Adlecreutz D, Wehtje E (2004) An enzymatic method for the synthesis of mixed-acid phosphatidylcholine. J Am Oil Chem Soc 81:553–557

    Article  Google Scholar 

  • Adlecreutz P, Lyberg AM, Adlecreutz D (2003) Enzymatic fatty acid exchange in glycerophospholipids. Eur J Lipid Sci Technol 105:638–645

    Article  Google Scholar 

  • Aura A-M, Forssell P, Mustranta A, Poutanen K (1995) Transesterification of soy lecithin by lipase and phospholipase. J Am Oil Chem Soc 72:1375–1379

    Article  CAS  Google Scholar 

  • Berry EJ, Hirsch J (1986) Does dietary linolenic acid influence blood pressure? Am J Clin Nutr 44:36–340

    Google Scholar 

  • Bigger JT, El-Sherif T (2001) Polyunsaturated fatty acids and cardiovascular events. A fish tale. Circulation 103:623–625

    PubMed  CAS  Google Scholar 

  • Doig SD, Diks RMM (2003) Toolbox for exchanging constituent fatty acids in lecithin. Eur J Lipid Sci Technol 105:368–376

    Article  CAS  Google Scholar 

  • Guo Z, Vikbjerg AF, Xu X (2005) Enzymatic modification of phospholipids for functional applications and human nutrition. Biotechnol Adv 23:203–259

    Article  PubMed  CAS  Google Scholar 

  • Hara S, Hasuo H, Nakasato M et al (2002) Modification of soybean phospholipids by enzymatic transacylation. J Oleo Sci 51:417–421

    CAS  Google Scholar 

  • Haraldsson GG, Thorarensen A (1999) Preparation of phospholipids highly enriched with n-3 polyunsaturated fatty acids by lipase. J Am Oil Chem Soc 76:1143–1149

    Article  CAS  Google Scholar 

  • Mead JF, Alfin-Slater RB, Howton DR, Popjak G (1986) Lipids: chemistry, biochemistry and nutrition. Plenum Press, New York

    Google Scholar 

  • Peng L, Xu X, Mu H et al (2002) Production of structured phospholipids by lipase-catalyzed acidolysis: optimization using response surface methodology. Enzyme Microb Technol 31:523–532

    Article  CAS  Google Scholar 

  • Reddy JRC, Vijeeta T, Karuna MSL et al (2005) Lipase-catalyzed preparation of palmitic and stearic acid-rich phosphatidylcholine. J Am Oil Chem Soc 82:727–730

    Article  CAS  Google Scholar 

  • Svenson I, Adelcreutz P, Mattiasson B et al (1993) Phase behaviour of aqueous systems of enzymatically modified phosphatidylcholines with one hexadecyl and one hexyl or octyl chain. Chem Phys Lipids 66:195–197

    Article  Google Scholar 

  • Vikbjerg A, Mu H, Xu X (2005a) Lipase-catalyzed acyl exchange of soybean phosphatidylcholine in n-hexane: a critical evaluation of both acyl incorporation and product recovery. Biotechnol Prog 21:397–404

    Article  PubMed  CAS  Google Scholar 

  • Vikbjerg AF, Peng L, Mu H, Xu X (2005b) Continuous production of structured phospholipids in a packed bed reactor with lipase from Thermomyces lanuginosa. J Am Oil Chem Soc 82:237–242

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Ministry of Science and Higher Education, Grant no R05 049 02.

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Correspondence to A. Chojnacka.

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Chojnacka, A., Gładkowski, W., Kiełbowicz, G. et al. Enzymatic enrichment of egg-yolk phosphatidylcholine with α-linolenic acid. Biotechnol Lett 31, 705–709 (2009). https://doi.org/10.1007/s10529-009-9915-6

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  • DOI: https://doi.org/10.1007/s10529-009-9915-6

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