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A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

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Abstract

Insect cell expression systems are widely used to produce active recombinant proteins. Here, we have developed a high-level expression vector containing a selectable marker for continuous production of recombinant proteins in insect cells. The plasmid, pXIHAbla, developed in this study, established a polyclonal cell line 8 days shorter than pXINSECT-DEST38 and pBmAneo. In addition, pXIHAbla exhibited an approximately fivefold higher average enhanced GFP expression level and approximately a twofold higher bionanocapsule secretion level than pXINSECT-DEST38. Using this plasmid, insect cells that highly express active proteins have been easily established.

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Acknowledgements

This study was supported by Grants-in-Aid for Research on Advanced Medical Technology from the Ministry of Health, Labor and Welfare.

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Authors and Affiliations

  1. Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada-Ku, Kobe, 657-8501, Japan

    Takuya Shishido

  2. Department of Chemical Science and Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan

    Naoya Kurata & Myung Eui Yoon

  3. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan

    Tsutomu Tanaka & Hideki Fukuda

  4. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan

    Hideki Yamaji & Akihiko Kondo

Authors
  1. Takuya Shishido
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  2. Naoya Kurata
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  3. Myung Eui Yoon
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  4. Tsutomu Tanaka
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  5. Hideki Yamaji
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  6. Hideki Fukuda
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  7. Akihiko Kondo
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Correspondence to Akihiko Kondo.

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Shishido, T., Kurata, N., Yoon, M.E. et al. A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells. Biotechnol Lett 31, 623–627 (2009). https://doi.org/10.1007/s10529-009-9910-y

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  • DOI: https://doi.org/10.1007/s10529-009-9910-y

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