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Relationship between glucose catabolism and xanthan production in Xanthomonas oryzae pv. oryzae

Biotechnology Letters volume 32, pages 527–531 (2010)Cite this article

Abstract

Two genes involved in central carbon metabolism were inactivated to modulate intracellular glucose 6-phosphate and to evaluate its effects on xanthan production in wild-type Xanthomonas oryzae pv. oryzae. Upon the inactivation of the phosphogluconate dehydratase gene (edd), intracellular glucose 6-phosphate increased from 0.05 to 1.17 mmol/g (dry cell wt). This was accompanied by increased xanthan production of up to 2.55 g/l (culture medium). In contrast, inactivation of 6-phosphogluconate dehydrogenase gene (gndA) did not influence intracellular glucose 6-phosphate nor xanthan production. The intracellular availability of glucose 6-phosphate is proposed as a rate-limiting factor in xanthan production, and it may be possible to increases production of xanthan by modulating the activities of enzymes in central carbon metabolism.

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Acknowledgments

This research was supported by the Agenda Program (Grant 20090101 060 026 001 02 00) funded by the Rural Development Administration, Republic of Korea, and in part by the Sangji University Research Fund 2008. The labGENE Inc. was supported by a grant from the Gangwon Technopark.

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Affiliations

  1. Division of Animal Science and Biotechnology, Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea

    Sang-Yoon Kim & Jae-Yong Cho

  2. Microbial Genetics Division, National Institute of Agricultural Biotechnology, 225 Seodun-dong, Suwon, 441-707, Korea

    Byoung-Moo Lee

  3. LabGene Inc., Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea

    Jae-Yong Cho

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  1. Sang-Yoon Kim
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  2. Byoung-Moo Lee
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Correspondence to Jae-Yong Cho.

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This paper is submitted to the section of Microbial and Enzyme Technology.

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Supplementary Table 1

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Supplementary Fig. 1

Growth of the X. oryzae wild-type and mutant strains in XOL medium supplemented with 0.4% (w/v) glucose. Symbols: closed circle, wild-type; open circle, the gnd mutant; closed square, the edd mutant strains. OD600, optical density at 600 nm. The arrows indicate the optical densities at which samples were withdrawn for enzyme assays and quantification of intracellular glucose 6-phosphate. Three independent extractions for each growth phase were performed (TIF 61 kb)

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Kim, SY., Lee, BM. & Cho, JY. Relationship between glucose catabolism and xanthan production in Xanthomonas oryzae pv. oryzae . Biotechnol Lett 32, 527–531 (2010). https://doi.org/10.1007/s10529-009-0193-0

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  • DOI: https://doi.org/10.1007/s10529-009-0193-0

Keywords

  • Central carbon metabolism
  • Glucose 6-phosphate
  • Rate-limiting step
  • Xanthomonas oryzae pv. oryzae
  • Xanthan