Conversion of α-amyrin into centellosides by plant cell cultures of Centella asiatica
- 270 Downloads
Plant cell cultures of Centella asiatica produce small quantities of centellosides: madecassosid > asiaticosid > madecassic acid > asiatic acid. To obtain a more efficient production system of these bioactive triterpenoid compounds, we developed a process where the substrate, α-amyrin, was converted into centellosides by cell suspensions of C. asiatica. When α-amyrin in acetone was added at 0.01 mg/ml−1 to the culture medium, together with the permeabilizing agent DMSO, after 7 days nearly 50% had penetrated the plant cells, of which almost 84% was transformed into centellosides. The system therefore efficiently converts α-amyrin into centellosides, thus opening a new possibility for the production of these compounds.
Keywordsα-Amyrin Asiaticoside Centella asiatica Madecassoside
This work was supported in part by DGPA, CONACYT and by grants from the Spanish “Ministerio de Ciencia e Innovación” (BIO2008-01210) and the “Generalitat de Catalunya” (2009SGR1217). Dr. Hernandez-Vazquez is grateful for her research grant from CONACYT.
- Kreis W, Hoelz H, May U, Reibhard E (1990) Storage of cardenolides in Digitalis lanata cells. Effect of dimethyl sulfoxide (DMSO) on cardenolide uptake and release. Plant Cell Tiss Organ Cult 20:191–199Google Scholar
- Mangas S, Moyano E, Hernandez-Vazquez L, Bonfill M (2009) Centella asiatica (L) Urban: An updated approach. In: Palazon J, Cusido RM (eds) Plant secondary terpenoids. Research Signpost, Trivandrum, pp 55–74Google Scholar