Biotechnology Letters

, Volume 32, Issue 2, pp 315–319 | Cite as

Conversion of α-amyrin into centellosides by plant cell cultures of Centella asiatica

  • Liliana Hernandez-Vazquez
  • Mercedes Bonfill
  • Elisabeth Moyano
  • Rosa M. Cusido
  • Arturo Navarro-Ocaña
  • Javier Palazon
Original Research Paper


Plant cell cultures of Centella asiatica produce small quantities of centellosides: madecassosid > asiaticosid > madecassic acid > asiatic acid. To obtain a more efficient production system of these bioactive triterpenoid compounds, we developed a process where the substrate, α-amyrin, was converted into centellosides by cell suspensions of C. asiatica. When α-amyrin in acetone was added at 0.01 mg/ml−1 to the culture medium, together with the permeabilizing agent DMSO, after 7 days nearly 50% had penetrated the plant cells, of which almost 84% was transformed into centellosides. The system therefore efficiently converts α-amyrin into centellosides, thus opening a new possibility for the production of these compounds.


α-Amyrin Asiaticoside Centella asiatica Madecassoside 



This work was supported in part by DGPA, CONACYT and by grants from the Spanish “Ministerio de Ciencia e Innovación” (BIO2008-01210) and the “Generalitat de Catalunya” (2009SGR1217). Dr. Hernandez-Vazquez is grateful for her research grant from CONACYT.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Liliana Hernandez-Vazquez
    • 1
  • Mercedes Bonfill
    • 1
  • Elisabeth Moyano
    • 2
  • Rosa M. Cusido
    • 1
  • Arturo Navarro-Ocaña
    • 3
  • Javier Palazon
    • 1
  1. 1.Laboratori de Fisiologia Vegetal, Facultat de FarmaciaUniversitat de BarcelonaBarcelonaSpain
  2. 2.Departament de Ciencies Experimentals i de la SalutUniversitat Pompeu FabraBarcelonaSpain
  3. 3.Departamento de Alimentos y Biotecnologia, Facultad de Quimica E-UNAMCd. UniversitariaMexicoMexico

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