Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba

Abstract

Treatment of suspension cells of Ginkgo biloba with fungal endophytes resulted in accumulation of flavonoids, increased abscisic acid (ABA) production and activation of phenylalanine ammonia-lyase (PAL). Fluridone, an inhibitor of ABA biosynthesis, was effective in inhibiting fungal endophytes-induced ABA biosynthesis, increase of PAL activity and flavonoids accumulation. Moreover, exogenous application of ABA enhanced PAL activity and increased accumulation of flavonoids in G. biloba cells with or without fungal endophytes elicitor. These finding suggest a causal relationship between ABA release and both PAL activity and flavonoid accumulation under fungal endophytes treatment and that ABA is involved in fungal endophytes-induced flavonoids accumulation in this plant.

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Acknowledgments

This work is supported by the research fund for the doctoral degree scholars of Taishan Medical University (No. 2005-04).

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Correspondence to Gangping Hao.

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Gangping Hao and Xihua Du contributed equally to this paper.

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Hao, G., Du, X., Zhao, F. et al. Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba . Biotechnol Lett 32, 305–314 (2010). https://doi.org/10.1007/s10529-009-0139-6

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Keywords

  • Abscisic acid
  • Flavonoid biosynthesis
  • Fungal endophytes
  • Ginkgo biloba
  • Phenylalanine ammonia-lyase