Continuous cultivation approach for fermentative succinic acid production from crude glycerol by Basfia succiniciproducens DD1

Abstract

A continuous cultivation process for the fermentative production of succinic acid from glycerol with the recently isolated bacterium Basfia succiniciproducens DD1 was developed. Crude glycerol (5.1 g l⁻¹) was used as C-source and NH₄OH as N-source and pH-control agent. The problem of wall growth was solved by transfers of the cultivation broth into an empty identical fermentor. The resulting continuous cultivation process was maintained for more than 80 days. Glycerol-limited steady states were established for dilution rates between 0.004 and 0.018 h⁻¹. Higher dilution rates resulted in glycerol accumulation. Succinic acid concentrations, productivities, yields and specific productivities increased with increasing dilution rates: at 0.018 h⁻¹ the highest values were 5.21 g l⁻¹, 0.094 g l⁻¹ h⁻¹, 1.02 g g⁻¹ and 0.375 g g⁻¹ h⁻¹, respectively.