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Construction and characterization of a novel staphylokinase variant with thrombin-inhibitory activity

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Abstract

Staphylokinase (SAK) is an effective thrombolysis agent for therapy of myocardial infarction. We have constructed a fusion SAK variant (SAK-HV) with a thrombin-binding domain composed of 12 amino acids from hirudin and expressed it in Escherichia coli and purified the resultant protein. SAK-HV maintained fibrinolytic activity similar to SAK and had anticoagulant activity attributable to its hirudin segment. Measurement of thrombin-binding activity in vitro demonstrated that SAK-HV possessed binding activity with thrombin while SAK did not. SAK-HV might thus be a more potent thrombolytic agent with anticoagulation property than SAK.

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Authors and Affiliations

  1. Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, 100850, China

    Min Wang, Yuanyuan Wang, Jinfeng Wang, Minji Zou, Shen Liu, Tao Xu, Xin Cai, Chen Wu, Jiaxi Wang & Donggang Xu

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  1. Min Wang
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  2. Yuanyuan Wang
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  3. Jinfeng Wang
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  4. Minji Zou
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  5. Shen Liu
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  6. Tao Xu
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  7. Xin Cai
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  8. Chen Wu
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  9. Jiaxi Wang
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  10. Donggang Xu
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Correspondence to Donggang Xu.

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Wang, M., Wang, Y., Wang, J. et al. Construction and characterization of a novel staphylokinase variant with thrombin-inhibitory activity. Biotechnol Lett 31, 1923–1927 (2009). https://doi.org/10.1007/s10529-009-0094-2

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  • DOI: https://doi.org/10.1007/s10529-009-0094-2

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