Abstract
The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.
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This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (M10641000067).
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Han, M., Park, I.S., Kim, S.H. et al. Design and characterization of a maltose binding protein-linked growth factor for matrix engineering. Biotechnol Lett 31, 1677–1684 (2009). https://doi.org/10.1007/s10529-009-0060-z
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DOI: https://doi.org/10.1007/s10529-009-0060-z