Abstract
Plant-specific bioactive compounds including feruloyltyramine (FT), 4-coumaroyltyramine (CT), and caffeoyltyramine (CaT) were simultaneously produced in Escherichia coli by heterologous expression of two biosynthetic genes encoding 4-coumarate:coenzyme A ligase and tyramine N-hydroxycinnamoyltransferase (THT) cloned from Arabidopsis thaliana and pepper, respectively. Simultaneous supplementation of substrates to the recombinant E. coli resulted in the secretion of multiple tyramine derivatives into the medium at high yield: CT (189 mg l−1), FT (135 mg l−1), CaT (40 mg l−1). In addition, the recombinant E. coli also produced, albeit at low concentration, a range of dopamine derivatives such as feruloyldopamine due to THT’s ability to accept dopamine as a substrate.
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Acknowledgements
This work was supported by the Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea. Also, this work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (R11-2001-09203001-0) and by the Biotechnology Research Institute of Chonnam National University.
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Kang, K., Park, M., Park, S. et al. Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli . Biotechnol Lett 31, 1469–1475 (2009). https://doi.org/10.1007/s10529-009-0032-3
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DOI: https://doi.org/10.1007/s10529-009-0032-3