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Large scale purification protocol for carnocin KZ 213 from Carnobacterium piscicola

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Abstract

Large scale purification of a class IIa bacteriocin has been developed to recover pure carnocin KZ 213 produced by Carnobacterium piscicola 213. Most previous protocols reported in the literature for the purification of small peptides have used reversed phase chromatography but scale-up is difficult. The first step of this new protocol is hydrophobic interaction chromatography, the second and third steps are cation exchange chromatography. The protocol leads to a complete recovery of carnocin KZ 213 with 95% purity and to a concentration factor of 83. From 10 l culture supernatant, 5.8 mg carnocin KZ 213 have been produced with a specific activity of 8,500 UA g−1. The protocol is easy to implement for larger volumes.

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Acknowledgments

The work accomplished during this study was undertaken with the technical support of J-P Defroyennes, G Foroni and L Garre and the financial support of the “Région Bruxelles Capitale” and the European Union for the co-financing of the Nanoimprint Programme. The authors acknowledge also Ruddy Wattiez (Université de Mons-Hainaut) for the characterization of carnocin KZ 213 by MS-analysis.

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Authors and Affiliations

  1. Institut Meurice, Unité de BioTechnologie, Campus CERIA, Av. E. Gryson 1, 1070, Brussels, Belgium

    C. Saint-Hubert & A. Durieux

  2. Meurice R&D asbl, Av. E. Gryson 1, 1070, Brussels, Belgium

    E. Bodo

  3. Eurobiotec sa, Allée de la Recherche 4, 1070, Brussels, Belgium

    J.-P. Simon

Authors
  1. C. Saint-Hubert
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  2. A. Durieux
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  3. E. Bodo
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  4. J.-P. Simon
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Corresponding author

Correspondence to C. Saint-Hubert.

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Saint-Hubert, C., Durieux, A., Bodo, E. et al. Large scale purification protocol for carnocin KZ 213 from Carnobacterium piscicola . Biotechnol Lett 31, 519–523 (2009). https://doi.org/10.1007/s10529-008-9897-9

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  • DOI: https://doi.org/10.1007/s10529-008-9897-9

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