Skip to main content
SpringerLink
Log in

Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

An Erratum to this article was published on 01 April 2009

Abstract

Micromonospora inyoensis produces sisomicin (Sm), an aminoglycoside antibiotic. The gene cluster of sisomicin biosynthesis spanning ca. 47 kb consists of 37 ORFs encoding various proteins for sisomicin biosynthesis, regulation, resistance and transport. The comparative genetic studies on the biosynthetic genes of sisomicin and gentamicin (Gm) reveal a similar biosynthetic route and provide a framework for the future biosynthetic studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Al-Feel W, Ewad MJS, Herbert CJ, Akhtar M (1983) Mechanism and stereochemistry of the elaboration of the neosamine C ring in the biosynthesis of neomycins. Chem Commun 18–20

  • Bibb MJ, Janssen GR, Ward JM (1985) Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene 38:215–226

    Article  PubMed  CAS  Google Scholar 

  • Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V (2000) Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature 407:340–348

    Article  PubMed  CAS  Google Scholar 

  • Cortes J, Haydock SF, Roberts GA, Bevitt DJ, Leadlay PF (1990) An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea. Nature 348:176–178

    Article  PubMed  CAS  Google Scholar 

  • Furuya K, Hutchinson CR (1998) The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin. FEMS Microbiol Lett 168:243–249

    Article  PubMed  CAS  Google Scholar 

  • Kharel MK, Basnet DB, Lee HC, Lion K, Moon YH, Kim JJ, Woo JS, Sohng JK (2004) Molecular cloning and characterization of a 2-deoxystreptamine biosynthetic gene cluster in gentamicin-producing Micromonospora echinospora ATCC 15835. Mol Cells 18:71–78

    PubMed  CAS  Google Scholar 

  • Lakshmi N, Kumar AG (1989) In vitro comparative activity of kanamycin, gentamicin and sisomicin—a new aminoglycoside, against clinical isolates. Indian J Pathol Microbiol 32:207–212

    PubMed  CAS  Google Scholar 

  • Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM (1999) Aminoglycosides: activity and resistance. Antimicrob Agents Chemother 43:727–737

    PubMed  CAS  Google Scholar 

  • Nam G, Kim SH, Kim JH, Shin JH, Jang ES (2002) An efficient and selective 1-N-monoethylation of sisomicin: process development of netilmicin. Org Process Res Dev 6:78–81

    Article  CAS  Google Scholar 

  • Park JW, Hong JSJ, Parajuli N, Koh HS, Park SR, Lee MO, Lim SK, Yoon YJ (2007) Analytical profiling of biosynthetic intermediates involved in the gentamicin pathway of Micromonospora echinospora by high-performance liquid chromatography using electrospray ionization mass spectrometric detection. Anal Chem 79:4860–4869

    Article  PubMed  CAS  Google Scholar 

  • Rinehart KL Jr, Malik JM, Nystrom RS, Stroshane RM, Truitt ST, Taniguchi M, Rolls JP, Haak WJ, Ruff BA (1974) Biosynthetic incorporation of [1-13C]glucosamine and [6-13C]glucose into neomycin. J Am Chem Soc 96:2263–2265

    Article  CAS  Google Scholar 

  • Sambrook J, David WR (2001) Molecular cloning: a laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Tamegai H, Nango E, Kuwahara M, Yamamoto H, Ota Y, Kuriki H, Eguchi T, Kakinuma K (2002) Identification of l-glutamine: 2-deoxy-scyllo-inosose-aminotransferase required for the biosynthesis of butirosin in Bacillus circulans. J Antibiot 55:707–714

    PubMed  CAS  Google Scholar 

  • Testa RT, Tilley BC (1975) Biotransformation, a new approach to aminoglycoside biosynthesis. I. Sisomicin. J Antibiot 28:573–579

    PubMed  CAS  Google Scholar 

  • Unwin J, Standage S, Alexander D, Hosted T, Horan AC, Wellington EMH (2004) Gene cluster in Micromonospora echinospora ATCC15835 for the biosynthesis of the gentamicin C complex. J Antibiot 57:436–445

    PubMed  CAS  Google Scholar 

Download references

Acknowledgement

We thank Prof. Chun-Bao Zhu, Shanghai Institute of Pharmaceutical Industry, for helpful discussion. This work was supported in part by grants from the National Natural Science Foundation of China (No. 30801449), the 43rd China Postdoctor Foundation (No. 20080430677) and the 2008 Shanghai Postdoctor Foundation (No. 08R214145).

Author information

Authors and Affiliations

  1. College of Biological Science and Technology, Fuzhou University, 523 Gongye Rd, Fuzhou City, Fujian, China

    Wun-Rong Hong & Zhi-Hong Zeng

  2. Shanghai Health Creation Center for Biopharmaceuticals R&D, 200 Newton Rd, Shanghai, China

    Mei Ge, Li Zhu & Min-Yu Luo

  3. Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Rd, Shanghai, China

    Lei Shao & Dai-Jie Chen

Authors
  1. Wun-Rong Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mei Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhi-Hong Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Li Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Min-Yu Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lei Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dai-Jie Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dai-Jie Chen.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-009-9982-8

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hong, WR., Ge, M., Zeng, ZH. et al. Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis . Biotechnol Lett 31, 449–455 (2009). https://doi.org/10.1007/s10529-008-9887-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-008-9887-y

Keywords

Search

Navigation