Enhanced synthesis of l-threo-3,4-dihydroxyphenylserine by high-density whole-cell biocatalyst of recombinant l-threonine aldolase from Streptomyces avelmitilis
- 287 Downloads
l-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural β-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing l-threonine aldolase (l-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10°C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions.
Keywordsβ-Hydroxy amino acid l-threo-2,3-Dihydroxyphenylserine l-Threonine aldolase Whole-cell conversion
- Baik SH, Yoshioka H, Yukawa H, Harayama S (2007) Synthesis of l-threo-3,4-dihydroxyphenylserine (l-threo-DOPS) with thermostabilized low-specific l-threonine aldolase from Streptomyces coelicolor A3(2). J Microbiol Biotechnol 17:1325–1329Google Scholar
- Liu JQ, Odani M, Yasuoka T, Dairi T, Itoh N, Kataoka M, Shimizu S, Yamada H (2000a) Gene cloning and overexpression of low-specific d-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drug. Appl Microbiol Biotechnol 54:44–51PubMedCrossRefGoogle Scholar
- Ohashi N, Nagata S, Ishizumi K, Maeshima K (1984) Process for producing threo-3(3,4-dihydroxyphenyl)serine. European Patent 0084928Google Scholar