Biotechnology Letters

, Volume 31, Issue 2, pp 277–281 | Cite as

Gibberellin production and phosphate solubilization by newly isolated strain of Acinetobacter calcoaceticus and its effect on plant growth

  • Sang-Mo Kang
  • Gil-Jae Joo
  • Muhammad Hamayun
  • Chae-In Na
  • Dong-Hyun Shin
  • Hak Youn Kim
  • Jin-Kyu Hong
  • In-Jung LeeEmail author
Original Research Paper


Plant growth-promoting rhizobacteria with gibberellins (GA)-producing potential were isolated from soil and screened for plant growth promotion. A new strain, Acinetobacter calcoaceticus SE370, produced extracellular GA and also had phosphate solubilising potential. It produced 10 different gibberellins, including the bioactive GA1, GA3 and GA4 which were at, respectively, 0.45, 6.2 and 2.8 ng/100 ml. The isolate solubilised tricalcium phosphate and lowered pH of the medium during the process. Culture filtrates of the organism after growth on broth promoted growth of cucumber, Chinese cabbage and crown daisy.


Acinetobactercalcoaceticus Gibberellin production Phosphate-solubilization Plant growth promotion 



This research project was supported by the Korea Research Foundation Grant (KRF-2004-005-F00062).

Supplementary material

10529_2008_9867_MOESM1_ESM.doc (26 kb)
MOESM1 (DOC 27 kb)


  1. Adachi M, Sako Y, Ishida Y (1996) Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions. J Phycol 32:424–432CrossRefGoogle Scholar
  2. Amies CR (1967) A modified formula for the preparation of Stuart’s transport medium. Can J Public Health 58:296–300PubMedGoogle Scholar
  3. Cassán F, Lucangeli C, Bottini R et al (2001) Azospirillum spp. metabolize [17,17-2H2]gibberellin A20 to [17,17-2H2]gibberellin A1 in vivo in dry rice mutant seedlings. Plant Cell Physiol 42:763–767PubMedCrossRefGoogle Scholar
  4. Dobert RC, Rood SB, Blevins DG (1992) Gibberellins and the legume-Rhizobium symbiosis. I. Endogenous gibberellins of lima bean (Phaseolus lunatus L.) stems and nodules. Plant Physiol 98:221–224PubMedCrossRefGoogle Scholar
  5. Gaskin P, MacMillan J (1991) GC-MS of gibberellins and related compounds. Methodology and a library of reference spectra. Cantocks Enterprises, Bristol, UKGoogle Scholar
  6. Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117CrossRefGoogle Scholar
  7. Joo G-J, Kim Y-M, Lee I-J et al (2004) Growth promotion of red pepper plug seedlings and the production of gibberellins by Bacillus cereus, Bacillus macroides and Bacillus pumilus. Biotechnol Lett 26:487–491PubMedCrossRefGoogle Scholar
  8. Kpomblekou AK, Tabatabai MA (1994) Effect of organic acids on release of P from phosphate rocks. Soil Sci 158:442–443CrossRefGoogle Scholar
  9. Lee I-J, Foster KR, Morgan PW (1998) Photoperiod control of gibberellin levels and flowering in sorghum. Plant Physiol 116:1003–1010PubMedCrossRefGoogle Scholar
  10. Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Ecol 170:265–270CrossRefGoogle Scholar
  11. Nautiyal CS, Bhaduria S, Kumar P et al (2000) Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiol Lett 182:291–296PubMedCrossRefGoogle Scholar
  12. Peix A, Rivas-Boyero AA, Mateos PF et al (2002) Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol Biochem 33:103–110CrossRefGoogle Scholar
  13. Piccoli P, Masciarelli O, Bottini R (1999) Gibberellin production by Azospirillum lipoferum cultured in chemically-defined medium as affected by oxygen availability and water status. Symbiosis 27:135–146Google Scholar
  14. Rodríguez H, Fraga R (1999) Phosphate-solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339PubMedCrossRefGoogle Scholar
  15. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Sang-Mo Kang
    • 1
  • Gil-Jae Joo
    • 2
  • Muhammad Hamayun
    • 1
  • Chae-In Na
    • 1
  • Dong-Hyun Shin
    • 1
  • Hak Youn Kim
    • 3
  • Jin-Kyu Hong
    • 4
  • In-Jung Lee
    • 1
    Email author
  1. 1.Division of Plant Biosciences, College of Agriculture and Life SciencesKyungpook National UniversityDaeguSouth Korea
  2. 2.Institute of Agricultural Science and TechnologyKyungpook National UniversityDaeguSouth Korea
  3. 3.Department of Environmental Conservation and Disaster Prevention, College of EnvironmentKeimyung UniversityDaeguSouth Korea
  4. 4.Hankook EM Industry Co., LtdDaeguSouth Korea

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