Abstract
Biodegradable elastic poly(l-lactide-co-ε-caprolactone) (PLCL) (50:50) copolymer was blended with collagen (0.05, 0.1 and 0.2% w/w) in an acidic dioxane solution to form a collagen/PLCL hybrid material suitable for tissue engineering applications. Stability and dispersivity of collagen on collagen/PLCL hybrid films and collagen coated PLCL films under mechanical stress were determined by a collagen release test and water contact angle measurement. Hybrid films had a higher stability than collagen-coated PLCL films. Elastic recovery as well as high interconnectivity and uniform pore morphology of the hybrid scaffolds were not affected by the collagen concentration. Fibroblasts (NIH-3T3) cell culture test was performed for cell growth and viability evaluation. Collagen concentration had little affect on the initial cell adhesion after 4 h cell culture; but after 48 h cell culture, increased cell proliferation on the hybrid films was observed. The hybrid material can be applied as a scaffold for vessel and cartilage regeneration for mechano-active tissue engineering.
Similar content being viewed by others
References
Cai Q, Yang J, Bei J, Wang S (2002) A novel porous cells scaffold made of polylactide–dextran blend by combining phase-separation and particle-leaching techniques. Biomaterials 23:4483–4492
Chen G, Ushida T, Tateishi T (2001) Development of biodegradable porous scaffolds for tissue engineering. Mater Sci Eng C 17:63–69
Cohen S, Baño MC, Cima LG, Allcock HR, Vacanti JP, Vacanti CA, Langer R (1993) Design of synthetic polymeric structures for cell transplantation and tissue engineering. Clin Mater 13:3–10
Doillon CJ, Silver FH (1986) Collagen-based wound dressing: Effects of hyaluronic acid and fibronectin on wound healing. Biomaterials 7:3–8
Fischer SE, Liu X, Mao HO, Harden JL (2007) Controlling cell adhesion to surfaces via associating bioactive triblock proteins. Biomaterials 28:3325–3337
Friess W (2003) Collagen in drug delivery and tissue engineering. Adv Drug Deliv Rev 55:1529–1530
Guidoin R, Marceau D, Couture J, Rao TJ, Merhi Y, Roy PE, Faye DDL (1989) Collagen coatings as biological sealants for textile arterial prostheses. Biomaterials 10:156–165
Hasirci K, Lewandrowski K, Gresser JD, Wise DL, Trantolo DJ (2001) Versatility of biodegradable biopolymers: degradability and an in vivo application. J Biotechnol 86:135–150
Inoguchi H, Kwon IK, Inoue E, Takamizawa K, Maehara Y, Matsuda T (2006) Mechanical responses of a compliant electrospun poly(l-lactide-co-ε-caprolactone) small-diameter vascular graft. Biomaterials 27:1470–1478
Jeong SI, Kim BS, Kang SW, Kwon JH, Lee YM, Kim SH, Kim YH (2004) In vivo biocompatibilty and degradation behavior of elastic poly(l-lactide-co-ε-caprolactone) scaffolds. Biomaterials 25:5939–5946
Jeong SI, Kwon JH, Lim JI, Cho SW, Jung Y, Sung WJ, Kim SH, Kim YH, Lee YM, Kim BS, Choi CY, Kim SJ (2005) Mechano-active tissue engineering of vascular smooth muscle using pulsatile perfusion bioreactors and elastic PLCL scaffolds. Biomaterials 26:1405–1411
Kellomäki M, Niiranen H, Puumanen K, Ashammakhi N, Waris T, Törmälä P (2000) Bioabsorbable scaffolds for guided bone regeneration and generation. Biomaterials 21:2495–2505
Kim SS, Park HJ, Han J, Choi CY, Kim BS (2003) Renal tissue reconstitution by the implantation of renal segments on biodegradable polymer scaffolds. Biotechnol Lett 25:1505–1508
Lee J, Tae G, Kim YH, Park IS, Kim S-H, Kim SH (2008) The effect of gelatin incorporation into electrospun poly(l-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility. Biomaterials 29:1872–1879
Liu C, Xia Z, Czernuszka JT (2007) Design and development of three dimensional scaffolds for tissue engineering. Chem Eng Res Des 85:1051–1064
Lubiatowski P, Kruczynski J, Gradys A, Trzeciak T, Jaroszewski J (2006) Articular cartilage repair by means of biodegradable scaffolds. Transplant Proc 38:320–322
Nair LS, Laurencin CT (2007) Biodegradable polymers as biomaterials. Prog Polym Sci 32:762–798
Ma PX (2008) Biomimetic materials for tissue engineering. Adv Drug Deliv Rev 60:184–198
Ma Z, Gao C, Gong Y, Shen J (2005) Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor. Biomaterials 26:1253–1259
Reignier J, Huneault MA (2006) Preparation of interconnected poly(ε-caprolactone) porous scaffolds by a combination of polymer and salt particulate leaching. Polymer 47:4703–4717
Shen H, Hu X, Bei J, Wang S (2008) The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide). Biomaterials 29:2388–2399
Tuckwell D, Humphries M (1996) Integrin–collagen binding. Semin Cell Dev Biol 7:649–657
Yang Y, Magnay JL, Cooling L, Haj AJE (2002) Development of a ‘mechano-active’ scaffold for tissue engineering. Biomaterials 23:2119–2126
Yang Y, Gu X, Tan R, Hu W, Wang X, Zhang P, Zhang T (2004) Fabrication and properties of a porous chitin/chitosan conduit for nerve regeneration. Biotechnol Lett 26:1793–1797
Acknowledgements
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund: KRF-2006-311-E00091).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lim, J.I., Yu, B. & Lee, YK. Fabrication of collagen hybridized elastic PLCL for tissue engineering. Biotechnol Lett 30, 2085–2090 (2008). https://doi.org/10.1007/s10529-008-9808-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-008-9808-0