Mice were fed either 13 nm silver nanoparticles or 2–3.5 μm silver microparticles. The livers were then obtained after 3 days and subjected to a histopathological analysis. The nanoparticle-fed and microparticle-fed livers both exhibited lymphocyte infiltration in the histopathological analysis, suggesting the induction of inflammation. In vitro, a human hepatoma cell line (Huh-7) was treated with the same silver nanoparticles and microparticles. The mitochondrial activity and glutathione production were hardly affected. However, the DNA contents decreased 15% in the nanoparticle-treated cells and 10% in the microparticle-treated cell, suggesting a more potent induction of apoptosis by the nanoparticles. From a microarray analysis of the RNA from the livers of the nano- and micro-particle-fed mice, the expression of genes related to apoptosis and inflammation was found to be altered. These gene expression changes in the nanoparticle-treated livers lead to phenotypical changes, reflecting increased apoptosis and inflammation. The changes in the gene expression were confirmed by using a semi-quantitative RT-PCR.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Ali S, Robertson H, Wain JH, Isaacs JD, Malik G, Kirby JA (2005) A non-glycosaminoglycan-binding variant of CC chemokine ligand 7 (monocyte chemoattractant protein-3) antagonizes chemokine-mediated inflammation. J Immunol 175:1257–1266
Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013–2016
Cabrero A, Laguna JC, Vázquez M (2002) Peroxisome proliferator-activated receptors and the control of inflammation. Curr Drug Targets Inflamm Allergy 1:243–248
Child ES, Mann DJ (2006) The intricacies of p21 phosphorylation: protein/protein interactions, subcellular localization and stability. Cell Cycle 5:1313–1319
Colvin V (2003) The potential environmental impacts of engineered nanomaterials. Nat Biotechnol 21:1166–1170
Cookson MR, van der Brug M (2008) Cell systems and the toxic mechanism(s) of alpha-synuclein. Exp Neurol 209:5–11
Cui Y, Wei Q, Park H, Lieber C (2001) Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science 293:1289–1292
Garrido C, Brunet M, Didelot C, Zermati Y, Schmitt E, Kroemer G (2006) Heat shock proteins 27 and 70: anti-apoptotic proteins with tumorigenic properties. Cell Cycle 5:2092–2601
Hofmeister R, Khaled AR, Benbernou N, Rajnavolgyi E, Muegge K, Durum SK (1999) Interleukin-7: physiological roles and mechanisms of action. Cytokine Growth Factor Rev 10:41–60
Husein S, Hess K, Gearheart J, Geiss K, Schlager J (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–983
Klein I, Sarkadi B, Váradi A (1999) An inventory of the human ABC proteins. Biochim Biophys Acta 1461:237–262
Lew W, Lee E, Krueger JG (2004) Psoriasis genomics: analysis of proinflammatory (type 1) gene expression in large plaque (Western) and small plaque (Asian) psoriasis vulgaris. Br J Dermatol 150:668–676
Louahed J, Struyf S, Demoulin JB, Parmentier M, Van Snick J, Van Damme J, Renauld JC (2003) CCR8-dependent activation of the RAS/MAPK pathway mediates anti-apoptotic activity of I-309/CCL1 and vMIP-I. Eur J Immunol 33:494–501
Mendez-Enriquez E, Melendez Y, Martinez F, Baay G, Huerta-Yepez S, Gonzalez-Bonilla C, Fortoul TI, Soldevila G, García-Zepeda EA (2008) CDIP-2, a synthetic peptide derived from chemokine (C–C motif) ligand 13 (CCL13), ameliorates allergic airway inflammation. Clin Exp Immunol [Epub ahead of print]
Mukhopadhyay S, Peiser L, Gordon S (2004) Activation of murine macrophages by Neisseria meningitidis and IFN-gamma in vitro: distinct roles of class A scavenger and toll-like pattern recognition receptors in selective modulation of surface phenotype. J Leukoc Biol 76:577–584
Oberdorster E (2004) Manufactured nanomaterials (fullerenes, C60) induce oxidative stress in the brain of juvenile largemouth bass. Environ Health Perspect 112:1058–1062
Rowell DL, Eckmann L, Dwinell MB, Carpenter SP, Raucy JL, Yang SK, Kagnoff MF (1997) Human hepatocytes express an array of proinflammatory cytokines after agonist stimulation or bacterial invasion. Am J Physiol 273:G322–G332
Schultz DR, Harrington WJ Jr (2003) Apoptosis: programmed cell death at a molecular level. Semin Arthritis Rheum 32:345–369
Suh JS, DiLella DP, Moskovits M (1983) Surface-enhanced Raman spectroscopy of colloidal metal systems. J Phys Chem 87:1540–1544
Taton T, Mirkin C, Letsinger R (2000) Scanometric DNA array detection with nanoparticle probes. Science 289:1757–1760
This work was supported by KOSEF grant M10203000039-05M0300-03910.
About this article
Cite this article
Cha, K., Hong, HW., Choi, YG. et al. Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles. Biotechnol Lett 30, 1893–1899 (2008). https://doi.org/10.1007/s10529-008-9786-2
- Gene expression profile
- Mouse liver