Abstract
Enzyme biofuel cells utilizing glucose dehydrogenase as an anode enzyme were constructed. The glucose dehydrogenase is composed of a catalytic subunit, an electron transfer subunit, and a chaperon-like subunit. Cells, constructed using either a glucose dehydrogenase catalytic subunit or a glucose dehydrogenase complex, displayed power outputs that were dependent on the glucose concentration. The catalytic subunit in the anode maintained its catalytic activity for 24 h of operation. The biofuel cell which composed of glucose dehydrogenase complex functioned successfully even in the absence of an electron mediator at the anode cell. These results indicate the potential application of this thermostable glucose dehydrogenase for the construction of a compartment-less biofuel cell.
Similar content being viewed by others
References
Barton Scott C, Gallaway J, Atanassov P (2004) Enzymatic biofuel cells for implantable and microscale devices. Chem Rev 104:4867–4886
Bullen RA, Arnot TC, Lakemanc JB, Walsh FC (2006) Biofuel cells and their development. Biosens Bioelectron 21:2015–2045
Chen T, Barton SC, Binyamin G, Gao Z, Zhang Y, Kim H-H, Heller A (2001) A Miniature Biofuel Cell. J Am Chem Soc 123:8630–8631
Heller A (2004) Miniature biofuel cells. Phys Chem Chem Phys 6:209–216
Inose K, Fujikawa M, Yamazaki T, Kojima K, Sode K (2003) Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli. Biochim Biophys Acta 1645:133–138
Kakehi N, Yamazaki T, Tsugawa W, Sode K (2007) A novel wireless glucose sensor employing direct electron transfer principle based enzyme fuel cell. Biosens Bioelectron 22:2250–2255
Katz E, Willner I (2003) A Biofuel Cell with Electrochemically Switchable and Tunable Power Output. J Am Chem Soc 125:6803–6813
Katz E, Filanovsky B, Willner I (1999a) A biofuel cell based on two immiscible solvents and glucose oxidase and microperoxidase-11 monolayer-functionalized electrodes. N J Chem 23:481–487
Katz E, Willner I, Kotlyar AB (1999b) A non-compartmentalized glucose O2 biofuel cell by bioengineered electrode surfaces. J Electroanal Chem 479:64–68
Katz E, Bueckmann AF, Willner I (2001) Self-powered enzyme-based biosensors. J Am Chem Soc 123:10752–10753
Mano N, Heller A (2003) A miniature membraneless biofuel cell operating at 0.36 V under physiological conditions. J Electrochem Soc 150:A1136–A1138
Mano N, Mao F, Heller A (2003) Characteristics of a miniature compartment-less glucose-O2 biofuel cell and its operation in a living plant. J Am Chem Soc 125:6588–6594
Mano N, Mao F, Heller A (2004) A miniature membrane-less biofuel cell operating at +0.60 V under physiological conditions. ChemBioChem 5:1703–1705
Nakazawa Y, Yamazaki T, Tsugawa W, Ikebukuro K, Sode K (2003) Amperometric glucose sensor using thermostable co-factor binding glucose dehydrogenase. IEEJ Trans Sens Micromachines 123:185–189
Okuda J, Sode K (2004) PQQ glucose dehydrogenase with novel electron transfer ability. Biochem Bioph Res Co 314:793–797
Okuda J, Yamazaki T, Fukasawa M, Kakehi N, Sode K (2007) The application of engineered glucose dehydrogenase to a direct electron-transfer-type continuous glucose monitoring system and a compartmentless biofuel cell. Anal Lett 40:431–440
Sato F, Togo M, Kamrul Islam M, Matsue T, Kosuge J, Fukasaku N, Kurosawa S, Nishizawa M (2005) Enzyme-based glucose fuel cell using Vitamin K3-immobilized polymer as an electron mediator. Electrochem Commun 7:643–647
Sode K, Tsugawa W, Yamazaki T, Watanabe M, Ogasawara N, Tanaka M (1996) A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures. Enz Microbial Technol 19:82–85
Tsuya T, Ferri S, Fujikawa M, Yamaoka H, Sode K (2006) Cloning and functional expression of glucose dehydrogenase complex of Burkholderia cepacia in Escherichia coli. J Biotechnol 123:127–196
Willner I, Katz E, Patolsky F, Buckmann AF (1998) Biofuel cell based on glucose oxidase and microperoxidase-11 monolayer-functionalized electrodes. J Chem Soc, Perkin Trans 2:1817–1822
Yamaoka H, Ferri S, Fujikawa M, Sode K (2004) Essential role of the small subunit of thermostable glucose dehydrogenase from Burkholderia cepacia. Biotechnol Lett 26:1757–1761
Yamazaki T, Tsugawa W, Sode K (1999a) Subunit analyses of a novel thermostable glucose dehydrogenase showing different temperature properties according to its quaternary structure. Appl Biochem Biotechnol 77–79:325–335
Yamazaki T, Tsugawa W, Sode K (1999b) Increased thermal stability of glucose dehydrogenase by cross-linking chemical modification. Biotechnol Lett 21:199–202
Yuhashi N, Tomiyama M, Okuda J, Igarashi S, Ikebukuro K, Sode K (2005) Development of a novel glucose enzyme fuel cell system employing protein engineered PQQ glucose dehydrogenase. Biosens Bioelectron 20:2145–2150
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Okuda-Shimazaki, J., Kakehi, N., Yamazaki, T. et al. Biofuel cell system employing thermostable glucose dehydrogenase. Biotechnol Lett 30, 1753–1758 (2008). https://doi.org/10.1007/s10529-008-9749-7
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-008-9749-7