Skip to main content

Advertisement

SpringerLink
Log in

Poly(vinyl alcohol) acetoacetate-based tissue adhesives are non-cytotoxic and non-inflammatory

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Polymer-based tissue adhesives composed of poly(vinyl alcohol) acetoacetate (PVOH acac) and cross-linking amines were investigated for their effects on cell survival and inflammatory cell activation using in vitro mouse cell cultures. Cytotoxicity of tissue adhesives was evaluated by placing adhesives in direct contact with 3T3 fibroblast cells. Tissue adhesives formulated from PVOH acac and 3-aminopropyltrialkoxysilane (APS) were non-cytotoxic to fibroblasts; adhesives formulated from PVOH acac and aminated poly(vinyl alcohol) (PVOH amine) were also non-cytotoxic to fibroblasts. In contrast, a commercial adhesive composed of 2-octyl cyanoacrylate was highly cytotoxic to fibroblasts. The inflammatory potential of tissue adhesives was evaluated by exposing J774 macrophage cells to adhesives, and measuring TNF-α release from macrophages. PVOH acac-based tissue adhesives did not elicit inflammatory TNF-α release from macrophages. These results suggest that PVOH acac-based tissue adhesives are non-cytotoxic and non-inflammatory. Such tissue adhesives represent a promising technology for a variety of medical applications, including surgical wound closure and tissue engineering, and the results are also significant in the design of in vitro cell culture systems to study biomaterials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

PVOH acac:

Poly(vinyl alcohol) acetoacetate

PVOH amine:

Aminated poly(vinyl alcohol)

APS:

3-Aminopropyltrimethoxysilane

LPS:

Lipopolysaccharide

References

  • Arthur SD (2006) Polymer-based tissue-adhesive for medical use. International Patent Application WO/2006/042169, 20 April 2006

  • Bhatia SK, Yetter AB (2007) Correlation of visual in vitro cytotoxicity ratings of biomaterials with quantitative in vitro cell viability measurements. Cell Biol Toxicol. doi: 10.1007/s10565-007-9040-z

  • Bruce J, Krukowski ZH, Al-Khairy G, Russell EM, Park KGM (2001) Systematic review of the definition and measurement of anastomotic leak after gastrointestinal surgery. Br J Surg 88:1157–1168

    Article  PubMed  CAS  Google Scholar 

  • Furst W, Banerjee A (2005) Release of glutaraldehyde from an albumin-glutaraldehyde tissue adhesive causes significant in vitro and in vivo toxicity. Ann Thorac Surg 79:1522–1528

    Article  PubMed  Google Scholar 

  • Haines-Butterick L, Rajagopal K, Branco M, Salick D, Rughani R, Pilarz M, Lamm MS, Pochan DJ, Schneider JP (2007) Controlling hydrogelation kinetics by peptide design for three-dimensional encapsulation and injectable delivery of cells. Proc Natl Acad Sci 104:7791–7796

    Article  PubMed  CAS  Google Scholar 

  • Hino M, Ishiko O, Honda KI, Yamane T, Ohta K, Takubo T, Tatsumi N (2000) Transmission of symptomatic parvovirus B19 infection by fibrin sealant used during surgery. Br J Haematol 108:194–195

    Article  PubMed  CAS  Google Scholar 

  • Kawamura M, Sawafuji M, Watanabe M, Horinouchi H, Kobayashi K (2002) Frequency of transmission of human parvovirus B19 infection by fibrin sealant used during thoracic surgery. Ann Thorac Surg 73:1098–1100

    Article  PubMed  Google Scholar 

  • Kim JC, Bassage SD, Kempski MH, del Cerro M, Park SB, Aquavella JV (1995) Evaluation of tissue adhesives in closure of scleral tunnel incisions. J Cataract Refract Surg 21:320–325

    PubMed  CAS  Google Scholar 

  • Leggat PA, Smith DR, Kedjarune U (2007) Surgical applications of cyanoacrylate adhesives: a review of toxicity. ANZ J Surg 77:209–213

    Article  PubMed  Google Scholar 

  • Papatheofanis FJ (1989) Cytotoxicity of alkyl-2-cyanoacrylate adhesives. J Biomed Mater Res 23:661–668

    Article  PubMed  CAS  Google Scholar 

  • Pickleman J, Watson W, Cunningham J, Fisher SG, Gamelli R (1999) The failed gastrointestinal anastomosis: an inevitable catastrophe? J Am Coll Surg 188:473–482

    Article  PubMed  CAS  Google Scholar 

  • Ratner BD, Hoffman AS, Schoen FJ, Lemons JE (eds) (1996) Biomaterials Science. Academic Press, New York

    Google Scholar 

  • Toriumi DM, Raslan WF, Friedman M, Tardy ME (1991) Variable histotoxicity of histoacryl when used in a subcutaneous site: an experimental study. Laryngoscope 101:339–343

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Thuy Mai, Ann Yetter, and Michael Panco for laboratory support work. We are also grateful to Dr. George Kodokian at DuPont and Dr. Surita Bhatia at the University of Massachusetts for helpful comments on this manuscript.

Author information

Authors and Affiliations

  1. Biochemical Sciences & Engineering, Central Research and Development, DuPont Experimental Station, E328/140B, P.O. Box 80328, Wilmington, DE, 19880, USA

    Sujata K. Bhatia & Samuel D. Arthur

Authors
  1. Sujata K. Bhatia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Samuel D. Arthur
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sujata K. Bhatia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bhatia, S.K., Arthur, S.D. Poly(vinyl alcohol) acetoacetate-based tissue adhesives are non-cytotoxic and non-inflammatory. Biotechnol Lett 30, 1339–1345 (2008). https://doi.org/10.1007/s10529-008-9709-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-008-9709-2

Keywords

Search

Navigation