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A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate

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Abstract

A novel class II 5-enoylpyruvylshikimate-3-phosphate synthase (EPSPS) was identified from Pseudomonas stutzeri A1501 by complementation of an Escherichia coli auxotrophic aroA mutant. The single amino acid substitution of serine (Ser) for asparagine (Asn)-130 of the A1501 EPSPS enhanced resistance to 200 mM glyphosate. The mutated EPSPS had a 2.5-fold increase for IC50 [glyphosate] value, a 2-fold increase for K i [glyphosate] value, but a K m [PEP] value similar to that of wild type. The effect of the single residue mutation on glyphosate resistance was also analyzed using a computer-based three-dimensional model.

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Acknowledgment

This work was supported by a grant from the Ministry of Science and Technology China (National Basic Research Program of China, 2007CB707805).

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Authors and Affiliations

  1. College of Biological Sciences, China Agricultural University, Beijing, 100094, P.R. China

    Aimin Liang

  2. Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081, P.R. China

    Aimin Liang, Jiying Sha, Wei Lu, Ming Chen, Liang Li, Yongliang Yan, Jin Wang, Shuzhen Ping, Wei Zhang & Min Lin

  3. College of Life Sciences, Sichuan Normal University, Chengdu, 610066, P.R. China

    Jiying Sha & Yiding Wang

  4. Biotechnology Research Center, Southwest University, Beibei, Chongqing, 400716, P.R. China

    Dan Jin

  5. Plant Genomics Research center, Mianyang Normal College, Mianyang, Sichuan, 621000, P.R. China

    Jin Wang

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  1. Aimin Liang
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  2. Jiying Sha
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  3. Wei Lu
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  4. Ming Chen
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  10. Wei Zhang
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  11. Yiding Wang
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  12. Min Lin
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Correspondence to Min Lin.

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Liang, A., Sha, J., Lu, W. et al. A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate. Biotechnol Lett 30, 1397–1401 (2008). https://doi.org/10.1007/s10529-008-9703-8

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  • DOI: https://doi.org/10.1007/s10529-008-9703-8

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