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Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans

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Abstract

Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l−1) and kinetin (0.2 mg l−1) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains ORS 571 and AA-SK-5 by means of imbibition. All treated calli had significant increases in protein content, total nitrogen and nitrogenase activity. Imbibition of ORS 571 had significant biochemical effect on the developing calli than AA-SK-5. The crop response study from the regenerated plantlets showed a positive correlation in yield than uninoculated control. The endophytic colonization was observed in all parts of the plants analyzed. Further, colonization was also confirmed by microtome sectioning.

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Acknowledgments

The authors thank Dr. P. Kalaiarasan for his assistance in microtome sectioning and photographing the images. The authors express their sincere gratitude to the National Agricultural Technology Project—Indian council of Agriculture Research (ICAR), New Delhi, India for the grant.

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Authors and Affiliations

  1. Department of Agricultural Microbiology, Horticultural College and Research Institute, Tamilnadu Agricultural University, Periyakulam, 625 604, Tamilnadu, India

    M. Senthilkumar

  2. Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea

    M. Madhaiyan

  3. Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore, 641 003, Tamilnadu, India

    M. Madhaiyan, S. P. Sundaram & H. Sangeetha

  4. National Biodiversity Authority of India, Chennai, Tamilnadu, India

    S. Kannaiyan

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  1. M. Senthilkumar
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  2. M. Madhaiyan
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  3. S. P. Sundaram
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  4. H. Sangeetha
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  5. S. Kannaiyan
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Correspondence to M. Senthilkumar.

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Senthilkumar, M., Madhaiyan, M., Sundaram, S.P. et al. Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans . Biotechnol Lett 30, 1477–1487 (2008). https://doi.org/10.1007/s10529-008-9693-6

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  • DOI: https://doi.org/10.1007/s10529-008-9693-6

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