Abstract
Polyethylenimine (PEI) is one of the most extensively studied non-viral vectors but its cytotoxicity limits its clinical value. PLGA nanospheres are biocompatible and can facilitate sustained release of plasmid DNA. This study compares the cytotoxicity and long-term transgene expression between PLGA nanosphere and PEI. PLGA nanospheres were significantly less cytotoxic than PEI at various concentrations. PLGA nanospheres induced significantly higher transgene expression in vitro for a longer duration (21 days) than PEI. We conclude that PLGA nanospheres have potential as gene delivery vehicles for use in gene therapy for diseases in which a long-term therapeutic gene expression regimen is necessary.
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Acknowledgements
This work was supported by a research fund from Seoul R&BD Program, South Korea. The authors thank Prof. Minhyung Lee at Hanyang University for kindly providing pCMV-Luc.
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Gwak, SJ., Kim, BS. Poly(lactic-co-glycolic acid) nanosphere as a vehicle for gene delivery to human cord blood-derived mesenchymal stem cells: comparison with polyethylenimine. Biotechnol Lett 30, 1177–1182 (2008). https://doi.org/10.1007/s10529-008-9676-7
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DOI: https://doi.org/10.1007/s10529-008-9676-7