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Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media

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Abstract

The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40°C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Noriho Kamiya, Yuichi Matsushita, Misa Hanaki, Kazunori Nakashima & Masahiro Goto

  2. Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Noriho Kamiya & Masahiro Goto

  3. Biotechnology Laboratory, Toyota Central R&D Lbs Inc, Aichi, Japan

    Mamiko Narita & Haruo Takahashi

Authors
  1. Noriho Kamiya
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  2. Yuichi Matsushita
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  3. Misa Hanaki
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  4. Kazunori Nakashima
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  5. Mamiko Narita
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  6. Masahiro Goto
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  7. Haruo Takahashi
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Correspondence to Noriho Kamiya.

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Kamiya, N., Matsushita, Y., Hanaki, M. et al. Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media. Biotechnol Lett 30, 1037–1040 (2008). https://doi.org/10.1007/s10529-008-9638-0

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  • DOI: https://doi.org/10.1007/s10529-008-9638-0

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