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Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor

Abstract

To develop an integrated process of CO2-fixation and H2 photoproduction by marine green microalga Platymonas subcordiformis, the impact of algal cells grown in CO2-supplemented air bubble column bioreactor was investigated on H2 photoproduction regulated by carbonylcyanide m-chlorophenylhrazone. Highest cell growth (3.85 × 106 cells ml−1), starch content (0.25 ± 0.08 mg per 10cells) and hydrogen production (50 ± 3 ml l−1) were achieved at 3% CO2-supplemented culture, which are respectively 1.4, 2.1, 1.5-fold of the air-supplemented culture. Improved H2 production correlated well with the increase in starch accumulation. In this process, the algal cells have been recycled for stable H2 production of 40–50 ml l−1 over five cycles.

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Acknowledgements

The financial supports are provided by National Basic R&D “973 Program’’ (2003CB214506), National High Technology R&D ‘‘863 Program’’ (2006AA05Z106), and “Innovation Fund” (KSCX2-YW-G-002) by the Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China.

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Correspondence to Wei Zhang.

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Guo, Z., Chen, Z., Zhang, W. et al. Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor. Biotechnol Lett 30, 877–883 (2008). https://doi.org/10.1007/s10529-008-9637-1

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  • DOI: https://doi.org/10.1007/s10529-008-9637-1

Keywords

  • Bioreactor
  • Carbon dioxide
  • Carbonylcyanide m-chlorophenylhydrazone (CCCP)
  • Hydrogen
  • Platymonas subcordiformis