In proteomic analysis, one of the major limitations is the detection of low-abundance proteins. To detect low-abundance RNA-binding proteins in mature dry seeds of rice, fractionation by single stranded DNA (ssDNA) affinity column chromatography was carried out before analysis by two-dimensional gel electrophoresis (2-DE). Proteomic analysis of the ssDNA-binding fraction revealed the existence of three types of RNA-binding proteins, including a K homology (KH) domain containing protein, a putative RNA-binding protein and a glycine-rich RNA-binding protein, in mature seeds. In addition, decreases in the putative RNA-binding protein and glycine-rich RNA-binding protein after absorbing water in seeds appear to be associated with seed germination.
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We are grateful to Prof. H. Fugo (Tokyo Univ. Agri. Tech.), Prof. Dr. T. Iino, Assoc. Prof. H. Sawada (Nihon Univ.) and Prof. H. Hirano (Yokohama City Univ.) for their interest and support. This study was supported in part by a grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan to M. K. (No. 19658002).
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Masaki, S., Yamada, T., Hirasawa, T. et al. Proteomic analysis of RNA-binding proteins in dry seeds of rice after fractionation by ssDNA affinity column chromatography. Biotechnol Lett 30, 955–960 (2008). https://doi.org/10.1007/s10529-007-9619-8
- KH domain
- Rice seed
- RNA-binding protein
- RNA recognition motif