Skip to main content
SpringerLink
Log in

The insecticidal crystal protein Cry2Ab10 from Bacillus thuringiensis: cloning, expression, and structure simulation

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The cry2Ab-type gene was cloned from Bacillus thuringiensis and designated as cry2Ab10. The recombinant Cry2Ab10 protein expressed in E. coli cells shows high toxicity against Plutella xylostella. The protein structure was constructed by homology modeling, and the receptor-binding sites were predicted by a molecular docking method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Boonserm P, Davis P, Ellar DJ, Li J (2005) Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications. J Mol Biol 348:363–382

    Article  PubMed  CAS  Google Scholar 

  • Knowles BH, Knight PJK, Ellar DJ (1991) N-acetyl galactosamine is part of the receptor in insect gut epithelia that recognizes an insecticidal protein from Bacillus thuringiensis. Proc Roy Soc Lond Ser B 245:31–35

    Article  CAS  Google Scholar 

  • Mizuki E, Ohba M, Akao T, Yamashita S, Saitoh H, Park YS (1999) Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis. J Appl Microbiol 86:477–486

    Article  PubMed  CAS  Google Scholar 

  • Sasaki J, Asano S, Hashimoto N, Lay BW, Hastowo S, Iizuka T (1997) Characterization of a cry2A gene cloned from an isolate of Bacillus thuringiensis serovar sotto. Curr Microbiol 35:1–8

    Article  PubMed  Google Scholar 

  • Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62:775–806

    PubMed  CAS  Google Scholar 

  • Song FP, Zhang J, Huang DF, Xie TJ, Yang ZW, Dai LY, Li GX (1998) Establishment of PCR-RFLP identification system of cry genes from Bacillus thuringiensis. Scientia Agricultura Sinica 31:13–18

    CAS  Google Scholar 

  • Wei JZ, Hale K, Carta L, Platzer E, Wong C, Fang SC, Aroian RV (2003) Bacillus thuringiensis crystal proteins that target nematodes. Proc Natl Acad Sci USA 100:2760–2765

    Article  PubMed  CAS  Google Scholar 

  • Yudina TG, Burtseva LI (1997) Activity of δ-endotoxins of four Bacillus thuringiensis subspecies against prokaryotes. Mikrobiol 66(1):30–36

    Google Scholar 

Download references

Acknowledgements

This work was partly supported by an open project from State Key Laboratory for Biology of Plant Diseases and Insect Pests, China. This work was also supported by NNSFC (National Natural Science Foundation of China, Grant No. 40601046), NCETFJ (Program for New Century Excellent Talents in Fujian Province University), and NSFFC (Natural Science Foundation of Fujian, China, Grant No. B0510011).

Author information

Authors and Affiliations

  1. Department of Bioengineering and Biotechnology, HuaQiao University, Key Laboratory of Industrial Biotechnology of Fujian Province Universities, Quanzhou, Fujian, 362021, China

    Yi Lin, Guangwei Fang & Fuying Cai

Authors
  1. Yi Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guangwei Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fuying Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yi Lin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, Y., Fang, G. & Cai, F. The insecticidal crystal protein Cry2Ab10 from Bacillus thuringiensis: cloning, expression, and structure simulation. Biotechnol Lett 30, 513–519 (2008). https://doi.org/10.1007/s10529-007-9572-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-007-9572-6

Keywords

Search

Navigation