Abstract
Cotton fabric samples were treated with a formulation of a total crude Trichoderma reesei cellulase in a two-step procedure. In the first step, samples were treated at a low liquor ratio by padding through the enzyme formulation at 21°C and 55°C with a wet pickup of 100% and batched for 12 h. The samples were then treated at a high liquor ratio (1:25) with an identical enzyme formulation at 55°C, with intensive agitation. The pre-treatment influenced the overall weight loss and rate of hydrolysis in samples, and the protein concentration in the liquor of the second step. The overall weight loss was 25–28% (w/w) in the two-step procedure compared to a weight loss of 22% (w/w) in the one-step batch hydrolysis.
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Acknowledgments
Authors gratefully acknowledge Christian-Doppler Research Society for financial funding and HTL und Versuchsanstalt Dornbirn, Austria for support in equipment, Roland Wohlgemuth and Reto Schuepbach, Fluka Chemie GmbH, Buchs, Switzerland for support with laboratory chemicals and Avinash P. Manian for proofreading and revision of English.
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Schimper, C.B., Ibanescu, C., Keckeis, R. et al. Advantages of a two-step enzymatic process for cotton–polyester blends. Biotechnol Lett 30, 455–459 (2008). https://doi.org/10.1007/s10529-007-9569-1
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DOI: https://doi.org/10.1007/s10529-007-9569-1