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Production of fructooligosaccharides by crude enzyme preparations of β-fructofuranosidase from Aureobasidium pullulans

Biotechnology Letters volume 30, pages 535–539 (2008)Cite this article

Abstract

Fructooligosaccharides (FOS) were produced from sucrose by using crude enzyme preparations of β-fructofuranosidases (FFases) obtained from sucrose-cultured cells of Aureobasidium pullulans DSM 2404. When the preparation mainly consisted of FFase I, that has high transfructosylating activity, the FOS yield was 62%. When the reaction was carried out with additional commercial glucose isomerase (GI) at an activity ratio of FFase and GI of 1:2, the maximum FOS yield reached 69%. This value was higher than those obtained previously using other Aureobasidium spp. (53–59%).

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Acknowledgements

We would like to thank Godo Shusei Co., LTD. for providing glucose isomerase (GODO AGI-S).

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  1. Jun Yoshikawa

    Present address: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan

Affiliations

  1. Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo-City, Chiba, 271-8510, Japan

    Jun Yoshikawa, Seigo Amachi, Hirofumi Shinoyama & Takaaki Fujii

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  1. Jun Yoshikawa
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  2. Seigo Amachi
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  3. Hirofumi Shinoyama
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Correspondence to Takaaki Fujii.

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Yoshikawa, J., Amachi, S., Shinoyama, H. et al. Production of fructooligosaccharides by crude enzyme preparations of β-fructofuranosidase from Aureobasidium pullulans . Biotechnol Lett 30, 535–539 (2008). https://doi.org/10.1007/s10529-007-9568-2

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  • DOI: https://doi.org/10.1007/s10529-007-9568-2

Keywords

  • Aureobasidium pullulans
  • β-fructofuranosidase
  • Fructooligosaccharides
  • Glucose isomerase
  • Prebiotic