Abstract
A two-step process is evaluated to separate the hexose component in wet milling corn fibers from the pentose component for production of value-added products. Corn fibers were first pretreated with hot water at 121°C for 1 h followed by glucoamylase hydrolysis to remove starch. The remaining solid was then treated with hot water at 140–170°C followed by an enzymatic hydrolysis to further separate the hexose and pentose components. After the second pretreatment, the enzymatic digestibility of cellulose was much better than that of arabinoxylan. As a result, up to 90% arabinoxylan in corn fibers was retained in a solid form after the enzyme hydrolysis, while most of the hexose components were removed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Doner LW, Hicks KB (1997) Isolation of hemicellulose from corn fiber by alkaline hydrogen peroxide extraction. Cereal Chem 74(2):176–181
Hespell RB (1998) Extraction and characterization of hemicellulose from corn fiber produced by corn wet-milling processes. J Agric Food Chem 46:2615–2619
Leathers TD, Gupta SC (1996) Saccharification of corn fiber using enzymes from Aureobasidium sp. Strain NRRL Y-2311-1. Appl Biochem Biotechnol 59:337–347
Leathers TD, Nunnally MS, Price NP (2006) Co-production of schizophyllan and arabinoxylan from corn fiber. Biotechnol Lett 28:623–626
Mosier NS, Hendrickson R, Brewer M et al (2005) Industrial scale-up of pH-controlled liquid hot water pretreatment of corn fiber for ethanol production. Appl Biochem Biotechnol 125:77–97
Raiagopalan S, Ponnampalam E, McCalla DSM (2005) Enhancing profitability of dry mill ethanol plants: process modeling and economics of conversion of degermed defibered corn to ethanol. Appl Biochem Biotechnol 120:37–50
Saha BC (2003) Hemicellulose bioconversion. J Ind Microbiol Biotechnol 30:279–291
Saha BC, Bothast RJ (1999) Pretreatment and enzymatic saccharification of corn fiber. Appl Biochem Biotechnol 77:65–77
Saha BC, Dien BS, Bothast RJ (1998) Fuel ethanol production from corn fiber: current status and technical prospects. Appl Biochem Biotechnol 70–72:115–125
Saulnier L, Marot C, Chanliaud E et al. (1995) Cell wall polysaccharide interactions in maize bran. Carbohydr Polym 26:279–287
Schell DJ, Riley CJ, Dowe N et al (2004) A bioethanol process development unit: initial operating experiences and results with a corn fiber feedstock. Bioresource Technol 91:179–188
Theander O, Westerlund EA (1986). Studies on dietary fiber. 3. Improved procedures for analysis of dietary fiber. J Agric Food Chem 34:330–336
Woo DH, Kim JK (2005) Method for preparing soluble dietary fiber from corn hull. US Patent 6,838,099, Jan 2005
Yang R, Zhang C, Feng H et al (2006) A kinetic study of xylan solubilization and degradation during corncob steaming. Biosyst Eng 93:375–382
Zhu Y, Kim TH, Lee YY et al (2006) Enzyme production of xylooligosaccharides from corn stover and corn cobs treated with aqueous ammonia. Appl Biochem Biotechnol 129–132:586–589
Acknowledgements
The authors would thank Tate & Lyle (Decatur, IL) for providing wet milling corn fibers. We are thankful to Dr. Penelope A. Patton at Tate & Lyle and Dr. Bruce S. Dien at the National Center for Agricultural Utilization Research (Peoria, IL) for their technical assistance in xylooligosaccharides analysis.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, B., Cheng, B. & Feng, H. Enriched arabinoxylan in corn fiber for value-added products. Biotechnol Lett 30, 275–279 (2008). https://doi.org/10.1007/s10529-007-9537-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-007-9537-9