Abstract
A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 μM to 1.6 mM with a detection limit of 3.1 μM glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (NO. 20675064), the Natural Science Foundation of Chongqing City (NO. CSTC-2004BB4149, 2005BB4100) and High Technology Project Foundation of Southwest University (XSGX 02).
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Yin, B., Yuan, R., Chai, Y. et al. Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode. Biotechnol Lett 30, 317–322 (2008). https://doi.org/10.1007/s10529-007-9534-z
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DOI: https://doi.org/10.1007/s10529-007-9534-z