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Biotechnology Letters

, Volume 30, Issue 2, pp 243–252 | Cite as

Aptameric enzyme subunit for homogeneous DNA sensing

  • Kazunori Ikebukuro
  • Wataru Yoshida
  • Koji Sode
Original Research Paper

Abstract

We have developed an aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution. The AES is an artificial enzyme subunit composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site. We connected a probe DNA to a thrombin-inhibiting aptamer at its 5′ or 3′ end. The inhibitory activity of the thrombin-inhibiting aptamer bearing the probe DNA decreased compared to that of the original aptamer; however, it recovered upon hybridization with the target DNA. Using this AES, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution.

Keywords

Aptameric enzyme subunit Enzyme-inhibiting aptamer Homogeneous DNA sensing 

Notes

Acknowledgements

This work was supported by Industrial Technology Research Grant Program in 2006 from New Energy and Industrial Technology Development Organization (NEDO) of Japan.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Biotechnology and Life ScienceTokyo University of Agriculture and TechnologyTokyoJapan

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