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Hematopoietic reconstitution of CD34+ cells grown in static and stirred culture systems in NOD/SCID mice

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Abstract

The hematopoietic reconstitution of cord blood (CB) CD34+ cells grown in static and stirred system was studied. Static cultures were better than stirred cultures for cell expansion. Engraftment of stirred-culture hematopoietic stem cells (HSCs) was higher than static-culture HSCs. Stirred-culture HSCs had better multilineage reconstitution ability and colony-forming ability than static-culture HSCs. Static cultures thus favor the expansion of HSCs and stirred cultures are more effective in preserving functional HSCs.

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Acknowledgement

This research work was supported by grants from key technologies R&D program of Shanghai (No. 05DZ19328).

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Authors and Affiliations

  1. The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Shi Yang, Haibo Cai, Huili Jin & Wen-Song Tan

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  1. Shi Yang
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  2. Haibo Cai
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  3. Huili Jin
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  4. Wen-Song Tan
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Correspondence to Wen-Song Tan.

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Yang, S., Cai, H., Jin, H. et al. Hematopoietic reconstitution of CD34+ cells grown in static and stirred culture systems in NOD/SCID mice. Biotechnol Lett 30, 61–65 (2008). https://doi.org/10.1007/s10529-007-9517-0

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  • DOI: https://doi.org/10.1007/s10529-007-9517-0

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