Skip to main content

Pichia pastoris expressing recombinant tilapia growth hormone accelerates the growth of tilapia

Biotechnology Letters volume 29, pages 1671–1676 (2007)Cite this article

Abstract

Growth manipulation of fish is an important task in aquatic biotechnology. The growth promoting effect of recombinant Pichia pastoris expressing tilapia growth hormone was demonstrated in red tilapia fry (Oreochromis sp.), which were immersed into water containing intact cells of the recombinant yeast. The weight increase of the treated group was 171% relative to the control group after 6 weeks.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Agellon LB, Emery CJ, Jones JM, Davies SL, Dingle AD, Chen TT (1988) Promoting of rapid growth of rainbow trout (Salmo gairdneri) by a recombinant fish growth hormone. Can J Fish Aquat Sci 45:146–151

    CAS  Google Scholar 

  • Baile CA, Della-Fera MA, McLaughlin C (1983) Performance and carcass quality of swine injected daily with bacterially-synthesized human growth hormone. Growth 47:225–236

    PubMed  CAS  Google Scholar 

  • Cheng WTK, Chen BC, Chiou ST, Chen CM (1998) Use of nonautologous microencapulated Þbroblasts in growth hormone gene therapy to improve growth of midget swine. Hum Gene Ther 9:1995–2003

    PubMed  CAS  Article  Google Scholar 

  • Chung CS, Etherton TD, Wiggins JP (1985) Stimulation of swine growth by porcine growth hormone. J Anim Sci 60:118–130

    PubMed  CAS  Google Scholar 

  • Cregg JM, Malden KR (1987) Development of yeast transformation system and construction of methanol utilization defective mutants of Pichia pastoris by disruption. In: Stewart G.G, Rusel I, Klein R.D, Heibsch R.R. (eds.), Biological Research on Industrial yeast, CRC Press, Boca Raton, FL, pp. 1–18

    Google Scholar 

  • Farmanfarmaian A, Sun LZ (1999) Growth hormone effects on essential amino acid absortion, muscle amino acid profile, N-retention and nutritional requirement of stripe bass hybrids. Genet Anal Biomol Eng 15:107–113

    Article  CAS  Google Scholar 

  • Guillén II, Lleonart R, Agramante A, Morales R, Morales A, Hernández CA, Vázquez MM, Díaz M, Herrera MT, Álvarez-Lajonchere L, Hernández O, de la Fuente J (1998) Physiological changes in the juvenile euryhaline teleost, the tilapia Oreochromis hornorum, injected with E. coli-derived homologous growth hormone. J Mar Biotechnol 6:142–151

    PubMed  Google Scholar 

  • Hertz Y, Tchelet A, Madar Z, Gertler A (1991) Absorption of bioactive human growth hormone after oral administration in the common carp and its enhancement by deoxycholate. J Comp Physiol 161:159–163

    CAS  Google Scholar 

  • Li YH, Bai JJ, Li XH, Ye X, Jian Q, Luo JR, Liang XF (2001) Expression of common carp growth hormone in yeast P. pastoris. Chin J Biochem Mol Biol 17:488–491

    CAS  Google Scholar 

  • Martínez E, García C, Grillo JM (1993) Rapid transformation of non-Saccharomyces yeast by electroporation. Biotechnol Tech 7:895–896

    Article  Google Scholar 

  • Moriyama S, Kawauchi H (1990) Growth stimulation of juvenile salmonids by immersion in recombinant salmon growth hormone. Nippon Suisan Gakkaishi 56:31–34

    CAS  Google Scholar 

  • Rodríguez M, Rubiera R, Penichet M, Montesino R, Cremata J, Falcón V, Sánchez G, Bringas R, Cordovés C, Valdés M, Lleonart R, Herrera L, de la Fuente J (1994) High level expression of the Boophilus microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle. J Biotechnol 33:135–146

    PubMed  Article  Google Scholar 

  • Sherwood NM, Harvey B (1986) Topical absorption of gonadotropin-releasing hormone (GnRH) in goldfish. Gen Comp Endocrinol 61:13–19

    PubMed  Article  CAS  Google Scholar 

  • Silverstein JT, Wolters WR, Shimizu M, Dickhoff WW (2000) Bovine growth hormone treatment of channel catfish: strain and temperature effects on growth, plasma IGF-I levels, feed intake and efficiency, and body composition. Aquaculture 190:7–88

    Article  Google Scholar 

  • Tsai HJ, Chi WK, Chang CC, Kuo TT, Chang CF (1993b) Enhancement of tilapia growth by dietary administration of recombinant yeast lysates as a supplement. J Fish Soc Taiwan 20:339–345

    Google Scholar 

  • Tsai HJ, Tseng CF, Kuo TT (1993a). Expression of rainbow trout growth hormone cDNA in yeast. Bull Inst Zool Acad Sin 32:162–170

    CAS  Google Scholar 

  • Tschopp JF, Sverlow R, Kosson R, Craig W, Grinna L (1987) High level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. Bio/Technol 5:1305–1308

    Article  CAS  Google Scholar 

  • Willard CL (2006) Welfare effects of the use of recombinant bovine somatotropin in the USA. J Dairy Res 14:1–12

    Google Scholar 

  • Yong V, González ME, Herrera L, Delgado J (1992) HIS-3 gene of Saccharomyces cerevisiae complement his mutation in yeast Pichia pastoris. Biotecnología Aplicada 9:55–61

    CAS  Google Scholar 

Download references

Acknowledgments

The authors thank J. García and L. Varas for helping with the bioreaction.

Author information

Affiliations

  1. Aquatics Biotechnology Project, Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, 10600, Cuba

    Jannel Acosta, Reynold Morales, Antonio Morales & Mario Pablo Estrada

  2. Ministry of fisheries, San José, Havana, Cuba

    Miriam Alonso

Authors
  1. Jannel Acosta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Reynold Morales
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Antonio Morales
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Miriam Alonso
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mario Pablo Estrada
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mario Pablo Estrada.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Acosta, J., Morales, R., Morales, A. et al. Pichia pastoris expressing recombinant tilapia growth hormone accelerates the growth of tilapia. Biotechnol Lett 29, 1671–1676 (2007). https://doi.org/10.1007/s10529-007-9502-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-007-9502-7

Keywords

  • Fish
  • Growth
  • Growth hormone
  • Immersion
  • Pichia pastoris
  • Tilapia