Abstract
We examined the expression of human cyclooxygenase-1 (COX-1) in Drososphila melanogaster S2 (S2) cells transformed with cDNAs encoding β1,4-galactosyltransferase (GalT) and Galβ1,4-GlcNAc α2,6-sialyltransferase (ST). Southern blot analysis indicated that multiple copies of the glycosyltransferases genes were integrated into the S2 cell genome. A lectin blot analysis also indicated that recombinant COX-1 from S2COX-1/GalT-ST cells contained the glycan residues of β1,4-linked galactose and α2,6-linked sialic acid. The specific peroxidase activity of recombinant sialylated COX-1 from S2COX-1/GalT-ST cells was 41,250 U mg−1, indicating an increase of approximately 22% compared with a non-sialylated control (33,850 U mg−1) from S2COX-1 cells.
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Acknowledgements
This work was supported by a grant (R01-2006-000-10635-0) from the Korea Science and Engineering Foundation (KOSEF), and by a grant from the KOSEF though the PMRC, Kyung Hee University.
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Chang, K.H., Lee, J.M., Hwang-Bo, J. et al. Expression of recombinant cyclooxygenase 1 in Drosophila melanogaster S2 cells transformed with human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase. Biotechnol Lett 29, 1803–1809 (2007). https://doi.org/10.1007/s10529-007-9489-0
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DOI: https://doi.org/10.1007/s10529-007-9489-0