Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli
The tdh (thermostable direct hemolysin) gene occurs in some strains of Vibrio species. All tdh genes are flanked by insertion sequence-like elements (ISV). All previous attempts have failed to detect transposition of these ISVs. In this work, we have built a transposition detection system in E. coli and succeeded in detecting the transposition of an insertion sequence-like element at a frequency of Kmr mutants of 7.2 × 10−6. A specific flanking sequence (5′-Py-Pu-3′) was found on either side of the target duplication.
KeywordsE. coli Insertion Transposase Transposition Vibrio anguillarum
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