Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli
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The tdh (thermostable direct hemolysin) gene occurs in some strains of Vibrio species. All tdh genes are flanked by insertion sequence-like elements (ISV). All previous attempts have failed to detect transposition of these ISVs. In this work, we have built a transposition detection system in E. coli and succeeded in detecting the transposition of an insertion sequence-like element at a frequency of Kmr mutants of 7.2 × 10−6. A specific flanking sequence (5′-Py-Pu-3′) was found on either side of the target duplication.
KeywordsE. coli Insertion Transposase Transposition Vibrio anguillarum
This work was supported by a grant from the National High-Tech Research Programs of China (No. 2001AA622020).
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