Biotechnology Letters

, Volume 29, Issue 12, pp 1951–1957 | Cite as

Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli

  • Na Liang
  • Haizhen Wu
  • Jiang Ye
  • Peng Xu
  • Yuanxing Zhang
  • Huizhan Zhang
Original Research Paper

Abstract

The tdh (thermostable direct hemolysin) gene occurs in some strains of Vibrio species. All tdh genes are flanked by insertion sequence-like elements (ISV). All previous attempts have failed to detect transposition of these ISVs. In this work, we have built a transposition detection system in E. coli and succeeded in detecting the transposition of an insertion sequence-like element at a frequency of Kmr mutants of 7.2 × 10−6. A specific flanking sequence (5′-Py-Pu-3′) was found on either side of the target duplication.

Keywords

E. coli Insertion Transposase Transposition Vibrio anguillarum 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Na Liang
    • 1
  • Haizhen Wu
    • 1
  • Jiang Ye
    • 1
  • Peng Xu
    • 1
  • Yuanxing Zhang
    • 1
  • Huizhan Zhang
    • 1
  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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