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Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD+ regeneration

Biotechnology Letters volume 29, pages 1857–1863 (2007)Cite this article

Abstract

By complementing a non-fermentative Escherichia coli (ldhA − pflB −) strain with the recombinant Zymomonas mobilis ethanol pathway (pdc, adhB), we evaluated the effect of different levels of enzymatic activity on growth rate demonstrating that there is a direct relationship between anaerobic growth rate and the total specific activity of pyruvate decarboxylase, which is the limiting enzyme of this specific fermentative NAD+ regenerating pathway. This relationship was proved to be useful to establish a selection strategy based on growth rate for the analysis of lctE libraries, which encode lactate dehydrogenase from Bacillus subtilis.

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Acknowledgements

This work was supported by CONACYT grants 138498, 50952, MOR-2004-C02-048; SAGARPA-2004-C01-224, and DGAPA-UNAM. The authors thank Eugenio López and Jorge Yañez from “Unidad de Síntesis y Secuenciación” (Instituto de Biotecnología, UNAM), Dr. Humberto Flores for technical assistance in the LDH work and Fernando González for HPLC support.

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  1. Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos, 62250, Mexico

    Consuelo Vázquez-Limón, Joel Vega-Badillo, Alfredo Martínez, Gabriela Espinosa-Molina, Guillermo Gosset, Xavier Soberón, Agustín López-Munguía & Joel Osuna

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  1. Consuelo Vázquez-Limón
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  2. Joel Vega-Badillo
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  3. Alfredo Martínez
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  4. Gabriela Espinosa-Molina
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  5. Guillermo Gosset
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  6. Xavier Soberón
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  7. Agustín López-Munguía
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  8. Joel Osuna
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Correspondence to Joel Osuna.

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Vázquez-Limón, C., Vega-Badillo, J., Martínez, A. et al. Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD+ regeneration. Biotechnol Lett 29, 1857–1863 (2007). https://doi.org/10.1007/s10529-007-9481-8

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  • DOI: https://doi.org/10.1007/s10529-007-9481-8

Keywords

  • Growth rate control
  • Lactate dehydrogenase
  • NAD+ regeneration
  • Non-fermentative Escherichia coli
  • Pyruvate decarboxylase