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Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures

Biotechnology Letters volume 29, pages 2001–2005 (2007)Cite this article

Abstract

Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3′-hydroxy-(S)-N-methylcoclaurine-4′-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.

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Acknowledgement

This work was financially supported by the Korea Science and Engineering Foundation (KOSEF)through the Advanced Environmental Biotechnology Research Center (ABERC) at POSTECH.

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Authors and Affiliations

  1. Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, 790-784, South Korea

    Hwa-Young Cho, Hong Soon Rhee & Jong Moon Park

  2. Chemical Engineering Department, Northeastern University, Boston, MA, 02115-5000, USA

    Carolyn W. T. Lee-Parsons

  3. Exelixis Plant Sciences, Portland, OR, 97224-7744, USA

    Sung-Yong H. Yoon

Authors
  1. Hwa-Young Cho
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  2. Carolyn W. T. Lee-Parsons
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  3. Sung-Yong H. Yoon
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  4. Hong Soon Rhee
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  5. Jong Moon Park
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Correspondence to Jong Moon Park.

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Cho, HY., Lee-Parsons, C.W.T., Yoon, SY.H. et al. Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Biotechnol Lett 29, 2001–2005 (2007). https://doi.org/10.1007/s10529-007-9469-4

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  • DOI: https://doi.org/10.1007/s10529-007-9469-4

Keywords

  • Benzophenanthridine alkaloid
  • Eschscholtzia californica
  • Methyl jasmonate
  • Salicylic acid
  • Signal transduction
  • Western blot