Abstract
The nodC gene from Mesorhizobium loti was cloned into E. coli, leading to production of chitin oligosaccharides (COs)—mainly penta-N-acetyl-chitopentaose. A two-step fermentation procedure was then developed which gave 930 mg CO/L with a productivity of 37 mg/l·h.
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Acknowledgements
This work was supported by a grant from the Ministry of Science and Technology of China (National Basic Research Program of China, 2007CB707803) and a grant from National Natural Science Foundation of China (30470399).
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Zhang, D., Wang, P.G. & Qi, Q. A two-step fermentation process for efficient production of penta-N-acetyl-chitopentaose in recombinant Escherichia coli . Biotechnol Lett 29, 1729–1733 (2007). https://doi.org/10.1007/s10529-007-9462-y
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DOI: https://doi.org/10.1007/s10529-007-9462-y