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Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants

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Abstract

To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.

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Authors and Affiliations

  1. The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, P.R. China

    Jie-Ying Bai, Yuan-Lei Hu & Zhong-Ping Lin

  2. Laboratory Animal Center of the Academy of Military Medical Science, Beijing, 100071, P.R. China

    Jie-Ying Bai, Lin Zeng, Shi-Chen Shang & Yan-Sheng Shi

  3. College of Plant Sciences, Jilin University, Changchun, 130062, P.R. China

    Yan-Fang Li

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  1. Jie-Ying Bai
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  2. Lin Zeng
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  3. Yuan-Lei Hu
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  4. Yan-Fang Li
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  5. Zhong-Ping Lin
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  6. Shi-Chen Shang
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  7. Yan-Sheng Shi
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Correspondence to Yuan-Lei Hu.

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Bai, JY., Zeng, L., Hu, YL. et al. Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants. Biotechnol Lett 29, 2007–2012 (2007). https://doi.org/10.1007/s10529-007-9438-y

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  • DOI: https://doi.org/10.1007/s10529-007-9438-y

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