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Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli

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An Erratum to this article was published on 28 December 2007

An Erratum to this article was published on 28 December 2007

Abstract

The gene encoding a single-chain, ribosome-inactivating protein (SCRIP) was cloned from bitter melon (Momordica charantia L.) leaves infected with the fungus, Sphaerotheca fuliginea, by RT-PCR. The ORF was 861 bp. The ribosome-inactivating protein was expressed in E. coli and, when purified, it inhibited the growth of the Sphaerotheca fuliginea in vitro. Northern blot analysis revealed that RIP transcripts rapidly accumulated in leaves 1-day post inoculation with Sphaerotheca fuliginea and reached a peak at 3 d. The expression pattern of RIP induced by methyl jasmonate and salicylic acid were different from that of pathogen-induced expression. Mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of RIP transcripts. Abscisic acid also induced transcription of RIPs. The signal compounds, ethylene and okadaic acid, induced a moderate accumulation of RIP transcripts.

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Authors and Affiliations

  1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Jun Xu & Hualin Wang

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Jun Xu

  3. The Laboratory of Toxicology, College of Public Health, Zheng Zhou University, Zheng Zhou, 450052, China

    Jianming Fan

Authors
  1. Jun Xu
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  2. Hualin Wang
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  3. Jianming Fan
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Correspondence to Jianming Fan.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-007-9625-x

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Xu, J., Wang, H. & Fan, J. Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli. Biotechnol Lett 29, 1605–1610 (2007). https://doi.org/10.1007/s10529-007-9433-3

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  • DOI: https://doi.org/10.1007/s10529-007-9433-3

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