Abstract
A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using Ni2+ resins. The recombinant NK, ∼30 kDa, retained fibrinolytic activity (60 U/ml). The integration of the EGFP expression cassette in the Bac-to-Bac system is thus an effective method for the expression and purification of recombinant NK protein in Spodoptera frugiperda insect cells.
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Acknowledgements
The authors thank Dr. Lunguang Y for excellent technical support. The authors also thank Dr. Yanyan Y, Guiming Z and Qi H from Hubei University, China, for critical reading through the manuscript and suggestions.
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Li, X., Wang, X., Xiong, S. et al. Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells. Biotechnol Lett 29, 1459–1464 (2007). https://doi.org/10.1007/s10529-007-9426-2
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DOI: https://doi.org/10.1007/s10529-007-9426-2