Abstract
To use nano-structured materials as a novel cell therapeutic agent, we have devised a novel method for the fabrication of nano-scaled 3D scaffolds consisting of heparin/poly(ethylenimine) (PEI) nanoparticles in a thermo-reversible hydrogel, attached via a layer-by-layer system. Bioassay results showed significant difference in DNA amount between groups. Specifically, groups with heparin/PEI nanoparticles had almost twice the glycosaminoglycan content per construct starting at day 7 as compared to controls. Gene expression of total collagen was evident in groups containing heparin/PEI nanoparticles.
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Acknowledgment
This work was supported by a Korea Research Foundation Grant (KRF2005-D00961).
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Na, K., Kim, S., Sun, B.K. et al. Blended construct consisting of thermo-reversible hydrogels and heparinized nanoparticles for increasing the proliferation activity of the rabbit chondrocyte in vivo test. Biotechnol Lett 29, 1447–1452 (2007). https://doi.org/10.1007/s10529-007-9422-6
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DOI: https://doi.org/10.1007/s10529-007-9422-6