Biotechnology Letters

, Volume 29, Issue 8, pp 1183–1187 | Cite as

Use of an industrial grade medium and medium enhancing effects on high cell density CO fermentation by Eubacterium limosum KIST612

  • In Seop Chang
  • Daehee Kim
  • Byung Hong Kim
  • Robert W. Lovitt
Original Research Paper


Studies were made on the composition of the growth medium to increase the cell concentration in a cell-recycled continuous culture (Eubacterium limosum KIST612) with carbon monoxide as a sole energy source using phosphate-buffered basal medium (PBBM) and modified PBBM. One of major limiting factors in PBBM might be nitrogen during the high cell density culture. This limitation could be overcome by increasing of inorganic nitrogen or yeast extract concentration in the medium. Anaerobic digester fluid, which could replace the organic nitrogen in PBBM, was used to develop an industrial grade medium for conversion of CO to multi-carbon compound.


Carbon monoxide Eubacterium limosum Industrial grade medium 



This work was supported by the Ministry of Science and Technology (International cooperation project, I-3-109) in Korea. Collaboration between Korean and British scientists was possible through the UK-Korean Joint Research Projects supplied by the British Council and the Korean Science and Engineering Foundation. This work was partly supported by grant from the MOST/KOSEF to the Environmental Biotechnology National Core Research Center (grant No: R15-203-012-02002-5), Korea.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • In Seop Chang
    • 1
    • 2
  • Daehee Kim
    • 1
  • Byung Hong Kim
    • 2
  • Robert W. Lovitt
    • 3
  1. 1.Department of Environmental Science and EngineeringGwangju Institute of Science and TechnologyGwangjuKorea
  2. 2.Water Environment & Remediation Research CentreKorea Institute of Science and TechnologySeoulKorea
  3. 3.Multidisciplinary Nanotechnology Centre, School of EngineeringSwansea UniversitySwanseaUK

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