Abstract
A system for the production of soluble interferon (IFN)-λ2 was developed by fusing the IFN-λ2, NusA protein, polyhistidine and S peptide genes and then expressing the fused product (Nus-His-S-tagged IFN-λ2) in Escherichia coli. The expressed fusion protein was purified by Ni-NTA affinity chromatography. The fusion tag was removed from recombinant IFN-λ2 by cleavage with enterokinase. N-Terminal sequencing confirmed the identity of the purified protein. When compared with commercial IFN-α2b, IFN-λ2 had similar antiviral activity. The production and characterization of biologically active IFN-λ2 will be beneficial for its potential role in clinical applications.
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This study was supported by the grant from the National Natural Science Foundation of China (No. 30671932).
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Li, M., Huang, D. Purification and characterization of prokaryotically expressed human interferon-λ2. Biotechnol Lett 29, 1025–1029 (2007). https://doi.org/10.1007/s10529-007-9357-y
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DOI: https://doi.org/10.1007/s10529-007-9357-y